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  • KD/KO Validated

GDF15 Polyclonal antibody

GDF15 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

IHC, WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 27455-1-AP

Print datasheet

Synonyms

GDF 15, GDF15, MIC 1, MIC1, NAG 1, NRG 1, NSAID activated gene 1 protein, NSAID regulated gene 1 protein, PDF, PLAB, Placental TGF beta, PTGFB



经过测试的应用

Positive WB detected inHT-1080 cells, HepG2 cells, human placenta tissue, LNCaP cells
Positive IHC detected inhuman placenta tissue, mouse placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27455-1-AP targets GDF15 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IHC, WB, ELISA
Cited Applications IF, IHC, WB
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat
Immunogen GDF15 fusion protein Ag26760 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name growth differentiation factor 15
Synonyms GDF 15, GDF15, MIC 1, MIC1, NAG 1, NRG 1, NSAID activated gene 1 protein, NSAID regulated gene 1 protein, PDF, PLAB, Placental TGF beta, PTGFB
Calculated Molecular Weight 34 kDa
Observed Molecular Weight 34 kDa
GenBank Accession NumberBC008962
Gene Symbol GDF15
Gene ID (NCBI) 9518
RRIDAB_2880875
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ99988
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Growth differentiation factor 15 (GDF15), also known as macrophage inhibitory cytokine-1 (MIC-1), is a protein of the transforming growth factor beta (TGFb) superfamily that regulates inflammatory and apoptotic pathways in injured tissues and during disease processes. GDF15 is a stress-induced cytokine and associated with hypoxia, inflammation and oxidative stress, it is also released from endothelial cells after stimulation with pro-inflammatory cytokines. GDF15 has been suggested as a target and biomarker for cardiovascular disease as it plays a cardioprotective role in the adult heart. GDF15 is highly expressed in the placenta. GDF15 is known to be involved in human embryo development and necessary for the maintenance of pregnancy. GDF15 is also produced by adipocytes in response to oxidative stress, a well-known factor associated with preeclampsia.

实验方案

Product Specific Protocols
WB protocol for GDF15 antibody 27455-1-APDownload protocol
IHC protocol for GDF15 antibody 27455-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,mouseWB

Gastroenterology

Hepatic Stellate Cells in Hepatocellular Carcinoma Promote Tumor Growth Via Growth Differentiation Factor 15 Production.

Authors - Yuta Myojin
  • KO Validated
humanWB

Nat Commun

Dual functions of SPOP and ERG dictate androgen therapy responses in prostate cancer.

Authors - Tiziano Bernasocchi
human,mouseWB,IHC

Cancer Lett

SULF2 enhances GDF15-SMAD axis to facilitate the initiation and progression of pancreatic cancer.

Authors - Ruizhe He
ratWB,IF

Oxid Med Cell Longev

Cyclic Polypeptide D7 Protects Bone Marrow Mesenchymal Cells and Promotes Chondrogenesis during Osteonecrosis of the Femoral Head via Growth Differentiation Factor 15-Mediated Redox Signaling.

Authors - Jiazheng Chen
humanWB

Cancers (Basel)

GDF15 Is an Eribulin Response Biomarker also Required for Survival of DTP Breast Cancer Cells.

Authors - Chiara Bellio
  • KD Validated
humanWB

Antioxidants (Basel)

Grape Seed Proanthocyanidins Induce Apoptosis and Cell Cycle Arrest of HepG2 Cells Accompanied by Induction of the MAPK Pathway and NAG-1.

Authors - Lihua Wang