验证数据展示
经过测试的应用
| Positive WB detected in | pig liver tissue, pig lung tissue |
| Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 3 publications below |
| WB | See 7 publications below |
| IHC | See 1 publications below |
产品信息
20713-1-AP targets GHR in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, pig samples.
| Tested Applications | WB, IHC, IF/ICC, ELISA Application Description |
| Cited Applications | WB, IHC |
| Tested Reactivity | human, pig |
| Cited Reactivity | human, pig, rabbit |
| Immunogen | Peptide 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | GH binding protein |
| Synonyms | Somatotropin receptor, Serum binding protein, Growth hormone-binding protein, GHBP, GH receptor |
| Calculated Molecular Weight | 72 kDa |
| Observed Molecular Weight | 120 kDa |
| GenBank Accession Number | NM_000163 |
| Gene Symbol | GHR |
| Gene ID (NCBI) | 2690 |
| RRID | AB_2878726 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P10912 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GH is a peptide hormone with many pleiotropic actions, including the regulation of metabolism, postnatal growth, cognition, immune, cardiac and renal systems and gut function (PMID: 26059750). GH exerts these actions primarily through alterations in gene expression, initiated by activation of its membrane receptor GHR and the resultant activation of the associated JAK2 and Src family kinases. GH receptor (GHR) is a member of the cytokine receptor family. Mutations in GHR gene have been associated with Laron syndrome, also known as the GH insensitivity syndrome (GHIS), a disorder characterized by short stature.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for GHR antibody 20713-1-AP | Download protocol |
| IHC protocol for GHR antibody 20713-1-AP | Download protocol |
| IF protocol for GHR antibody 20713-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Pharmacol Res Discovery of a novel megakaryopoiesis enhancer, ingenol, promoting thrombopoiesis through PI3K-Akt signaling independent of thrombopoietin. | ||
J Cell Mol Med GHR is involved in gastric cell growth and apoptosis via PI3K/AKT signalling.
| ||
Cell Signal The inhibition of GHR enhanced cytotoxic effects of etoposide on neuroblastoma. | ||
Front Cell Dev Biol One-step in vivo gene knock-out in porcine embryos using recombinant adeno-associated viruses |
