验证数据展示
经过测试的应用
| Positive WB detected in | pig liver tissue, pig lung tissue |
| Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 3 publications below |
| WB | See 7 publications below |
| IHC | See 1 publications below |
产品信息
20713-1-AP targets GHR in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, pig samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, pig |
| 文献引用反应性 | human, pig, rabbit |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | GH binding protein |
| 别名 | Somatotropin receptor, Serum binding protein, Growth hormone-binding protein, GHBP, GH receptor |
| 计算分子量 | 72 kDa |
| 观测分子量 | 120 kDa |
| GenBank蛋白编号 | NM_000163 |
| 基因名称 | GHR |
| Gene ID (NCBI) | 2690 |
| RRID | AB_2878726 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P10912 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GH is a peptide hormone with many pleiotropic actions, including the regulation of metabolism, postnatal growth, cognition, immune, cardiac and renal systems and gut function (PMID: 26059750). GH exerts these actions primarily through alterations in gene expression, initiated by activation of its membrane receptor GHR and the resultant activation of the associated JAK2 and Src family kinases. GH receptor (GHR) is a member of the cytokine receptor family. Mutations in GHR gene have been associated with Laron syndrome, also known as the GH insensitivity syndrome (GHIS), a disorder characterized by short stature.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for GHR antibody 20713-1-AP | Download protocol |
| IHC protocol for GHR antibody 20713-1-AP | Download protocol |
| IF protocol for GHR antibody 20713-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Pharmacol Res Discovery of a novel megakaryopoiesis enhancer, ingenol, promoting thrombopoiesis through PI3K-Akt signaling independent of thrombopoietin. | ||
J Cell Mol Med GHR is involved in gastric cell growth and apoptosis via PI3K/AKT signalling.
| ||
Cell Signal The inhibition of GHR enhanced cytotoxic effects of etoposide on neuroblastoma. | ||
Front Cell Dev Biol One-step in vivo gene knock-out in porcine embryos using recombinant adeno-associated viruses |
