验证数据展示
经过测试的应用
Positive WB detected in | A549 cells, HEK-293T cells, MCF-7 cells, SH-SY5Y cells, human placenta tissue, mouse kidney tissue |
Positive IP detected in | mouse brain tissue |
Positive IF/ICC detected in | SH-SY5Y cells, HepG2 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 1 publications below |
IF | See 1 publications below |
产品信息
11978-2-AP targets GNB4 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.
Tested Applications | WB, IF/ICC, IP, ELISA Application Description |
Cited Applications | WB, IF |
Tested Reactivity | human, mouse |
Cited Reactivity | human |
Immunogen | GNB4 fusion protein Ag2604 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | guanine nucleotide binding protein (G protein), beta polypeptide 4 |
Synonyms | HG2B, Guanine nucleotide-binding protein subunit beta-4, CMTD1F |
Calculated Molecular Weight | 340 aa, 38 kDa |
Observed Molecular Weight | 38 kDa |
GenBank Accession Number | BC000873 |
Gene Symbol | GNB4 |
Gene ID (NCBI) | 59345 |
RRID | AB_2109620 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q9HAV0 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
G protein subunit beta 4 (GNB4), is one of the heterotrimeric guanine nucleotide-binding proteins (G proteins), which integrate signals between receptors and effector proteins. GNB4 is important regulators of alpha subunits, as well as of certain signal transduction receptors and effectors.
实验方案
Product Specific Protocols | |
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WB protocol for GNB4 antibody 11978-2-AP | Download protocol |
IF protocol for GNB4 antibody 11978-2-AP | Download protocol |
IP protocol for GNB4 antibody 11978-2-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
BMC Med Helicobacter pylori-induced aberrant demethylation and expression of GNB4 promotes gastric carcinogenesis via the Hippo-YAP1 pathway | ||
Nat Commun Cross-linking of the endolysosomal system reveals potential flotillin structures and cargo |