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验证数据展示

  • WB analysis using 60317-1-Ig

    WB analysis using 60317-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 60317-1-Ig (GOT1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 60317-1-Ig

    IHC staining of human liver cancer using 60317-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60317-1-Ig (GOT1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 60317-1-Ig

    IHC staining of human liver cancer using 60317-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60317-1-Ig (GOT1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 60317-1-Ig

    IF Staining of HepG2 using 60317-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using GOT1 antibody (60317-1-Ig, Clone: 5A12E10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Featured Product
  • KD/KO Validated

GOT1 Monoclonal antibody

GOT1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 60317-1-Ig

Print datasheet

CloneNo. 5A12E10
Publications(2)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, chicken

Applications

WB, IHC, IF/ICC, ELISA and More (2)

Synonyms

CAT, GIG18, GOT1, Transaminase A

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inLNCaP cells, rat brain tissue, rat heart tissue, mouse brain tissue, K-562 cells, chicken brain, chicken heart
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

60317-1-Ig targets GOT1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat, chicken samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IF, IP
Tested Reactivity human, mouse, rat, chicken
Cited Reactivityhuman
Immunogen GOT1 fusion protein Ag7012 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name glutamic-oxaloacetic transaminase 1, soluble (aspartate aminotransferase 1)
Synonyms CAT, GIG18, GOT1, Transaminase A
Calculated Molecular Weight 46 kDa
Observed Molecular Weight 40-46 kDa
GenBank Accession NumberBC000498
Gene Symbol GOT1
Gene ID (NCBI) 2805
RRIDAB_2881428
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP17174
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Glutamate oxaloacetate transaminase 1 (GOT1) catalyzes the reversible reaction of L-aspartate and alpha-ketoglutarate into oxaloacetate and L-glutamate and plays a key role in carbon and nitrogen metabolism. GOT1 can potentially control the intracellular levels of reactive oxygen species (ROS) through NADPH synthesis and enhances tumor growth. GOT1 expression correlates with the growth of several tumors.

实验方案

Product Specific Protocols
WB protocol for GOT1 antibody 60317-1-IgDownload protocol
IHC protocol for GOT1 antibody 60317-1-IgDownload protocol
IF protocol for GOT1 antibody 60317-1-IgDownload protocol
FC protocol for GOT1 antibody 60317-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Cell Mol Med

HIF-2α regulates non-canonical glutamine metabolism via activation of PI3K/mTORC2 pathway in human pancreatic ductal adenocarcinoma.

Authors - Wenzhu Li
View Article
humanWB,IF,IP

Nat Cell Biol

De novo pyrimidine biosynthetic complexes support cancer cell proliferation and ferroptosis defence

Authors - Chuanzhen Yang
  • KD Validated
View Article