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GPR132 Polyclonal antibody
GPR132 Polyclonal Antibody for IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
IHC, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive IHC detected in | human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| IHC | See 1 publications below |
产品信息
17026-1-AP targets GPR132 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | IHC, ELISA |
| Cited Applications | WB, IHC |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Immunogen | GPR132 fusion protein Ag10325 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | G protein-coupled receptor 132 |
| Synonyms | G protein coupled receptor 132, G2 accumulation protein, G2A, GPR132 |
| Calculated Molecular Weight | 380 aa, 43 kDa |
| GenBank Accession Number | BC084546 |
| Gene Symbol | GPR132 |
| Gene ID (NCBI) | 29933 |
| RRID | AB_2113375 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UNW8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GPR132, also known as G2A (G2 accumulation protein), belongs to the G-protein coupled receptor (GPCR) 1 family. GPR132 is a DNA damage and stress inducible GPCR that blocks cells in G2/M. It functions at the G2/M checkpoint to delay mitosis and may serve as a mechanism for T and B cells and other cell types to slow their proliferation and repair damaged DNA to ensure proper replication (PMID: 9770487). Lysophosphatidylcholine (LPC) is a high affinity ligand for GPR132 (PMID: 15653487).
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for GPR132 antibody 17026-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Eur Acad Dermatol Venereol Lysophosphatidylcholine facilitates the pathogenesis of psoriasis through activating keratinocytes and T cells differentiation via glycolysis | ||
Front Oncol Lactate increases tumor malignancy by promoting tumor small extracellular vesicles production via the GPR81-cAMP-PKA-HIF-1α axis
| ||
J Hepatobiliary Pancreat Sci Partial characterization of proapoptotic action of biliary deteriorated lipids on biliary epithelial cells in pancreaticobiliary diseases. | ||
Nat Commun Lysophosphatidylserine induces necrosis in pressure overloaded male mouse hearts via G protein coupled receptor 34 |
