GPX4 Polyclonal antibody

GPX4 Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IF, ELISA and More (2)

Conjugate

Unconjugated

Cat no : 14432-1-AP

Print datasheet

Synonyms

GPX4



经过测试的应用

Positive WB detected inmouse testis tissue, rat testis tissue
Positive IF/ICC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14432-1-AP targets GPX4 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF, ELISA Application Description
Cited ApplicationsWB, IHC, IF, CoIP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, pig, chicken
Immunogen GPX4 fusion protein Ag5809 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name glutathione peroxidase 4 (phospholipid hydroperoxidase)
Synonyms GPX4
Calculated Molecular Weight 22 kDa
Observed Molecular Weight 20-23 kDa
GenBank Accession NumberBC022071
Gene Symbol GPX4
Gene ID (NCBI) 2879
RRIDAB_10898356
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP36969
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GPX4 (Phospholipid hydroperoxide glutathione peroxidase, mitochondrial) protects cells against membrane lipid peroxidation and cell death. Required for normal sperm development and male fertility.It has two isforms about 20KDa and 22KDa, respectively. GPX4 is a monomer,but it has a tendency to form higher mass oligomers (PMID:17630701). It presents primarily in testis.

实验方案

Product Specific Protocols
WB protocol for GPX4 antibody 14432-1-APDownload protocol
IF protocol for GPX4 antibody 14432-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Drug Resist Updat

Upregulation of CoQ shifts ferroptosis dependence from GPX4 to FSP1 in acquired radioresistance

Authors - Xu Lin
mouseWB

Biomaterials

Tumor microenvironment-initiated lipid redox cycling for efficient triple-negative breast cancer therapy

Authors - Tian-Jiao Zhou
chickenWB

J Hazard Mater

Mitochondrial miR-12294-5p regulated copper-induced mitochondrial oxidative stress and mitochondrial quality control imbalance by targeted inhibition of CISD1 in chicken livers

Authors - Gaolong Zhong
mouseIHC

Drug Des Devel Ther

Vancomycin Induced Ferroptosis in Renal Injury Through the Inactivation of Recombinant Glutathione Peroxidase 4 and the Accumulation of Peroxides

Authors - Xuedong Yin
mouseWB

Drug Des Devel Ther

Ketamine Attenuates Airway Inflammation via Inducing Inflammatory Cells Apoptosis and Activating Nrf2 Pathway in a Mixed-Granulocytic Murine Asthma Model

Authors - Shilin Xiao
mouseWB

Mater Today Bio

In situ injectable hydrogel-loaded drugs induce anti-tumor immune responses in melanoma immunochemotherapy.

Authors - Jiehan Li