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GSS Polyclonal antibody

GSS Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig, canine, hamster and More (1)

Applications

WB, IHC, IF/ICC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 15712-1-AP

Print datasheet

Synonyms

GSHS, GSH synthetase, GSH S, glutathione synthetase, Glutathione synthase



经过测试的应用

Positive WB detected inCHO cells, HEK-293 cells, mouse liver tissue, rat liver tissue
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMDCK cells
Planning an IHC experiment? We recommend our IHCeasy GSS Ready-To-Use IHC Kit. GSS primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15712-1-AP targets GSS in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig, canine, hamster samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IHC, IF
Tested Reactivity human, mouse, rat, pig, canine, hamster
Cited Reactivityhuman, mouse, rat, pig, chicken
Immunogen GSS fusion protein Ag8338 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name glutathione synthetase
Synonyms GSHS, GSH synthetase, GSH S, glutathione synthetase, Glutathione synthase
Calculated Molecular Weight 474 aa, 52 kDa
Observed Molecular Weight 52 kDa
GenBank Accession NumberBC007927
Gene Symbol GSS
Gene ID (NCBI) 2937
RRIDAB_2878171
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP48637
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for GSS antibody 15712-1-APDownload protocol
IHC protocol for GSS antibody 15712-1-APDownload protocol
IF protocol for GSS antibody 15712-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouse,humanWB,IHC,IF

ACS Nano

Engineered Extracellular Vesicle-Delivered CRISPR/Cas9 for Radiotherapy Sensitization of Glioblastoma

Authors - Xiao Liu
humanWB

Oxid Med Cell Longev

IMCA Induces Ferroptosis Mediated by SLC7A11 through the AMPK/mTOR Pathway in Colorectal Cancer.

Authors - Lei Zhang
mouseWB

Redox Biol

γ-glutamylcysteine exhibits anti-inflammatory effects by increasing cellular glutathione level.

Authors - Yang Yang
humanWB

Pharmacol Res

Phyto-sesquiterpene lactones DET and DETD-35 induce ferroptosis in vemurafenib sensitive and resistant melanoma via GPX4 inhibition and metabolic reprogramming.

Authors - Meng-Ting Chang
mouseWB

FASEB J

Taurine treatment prevents derangement of the hepatic γ-glutamyl cycle and methylglyoxal metabolism in a mouse model of classical homocystinuria: regulatory crosstalk between thiol and sulfinic acid metabolism.

Authors - Kenneth N Maclean
mouseWB

Cell Physiol Biochem

Effect of Type I Diabetes on the Proteome of Mouse Oocytes.

Authors - Guangjian Jiang