TIM3 Polyclonal antibody

TIM3 Polyclonal Antibody for FC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

FC, WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 11872-1-AP

Print datasheet

Synonyms

CD366, HAVcr 2, HAVCR2, KIM 3, T cell membrane protein 3, Tim 3, TIM3, TIM-3, TIMD 3, TIMD3



经过测试的应用

Positive WB detected inPC-3 cells, Daudi cells, Raji cells, RAW 264.7 cells
Positive FC detected inRaji cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Flow Cytometry (FC)FC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11872-1-AP targets TIM3 in WB, IHC, FC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications FC, WB, ELISA
Cited ApplicationsIHC, WB
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Immunogen TIM3 fusion protein Ag2459 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name hepatitis A virus cellular receptor 2
Synonyms CD366, HAVcr 2, HAVCR2, KIM 3, T cell membrane protein 3, Tim 3, TIM3, TIM-3, TIMD 3, TIMD3
Calculated Molecular Weight 301 aa, 33 kDa
Observed Molecular Weight 50-70 kDa, 33 kDa
GenBank Accession NumberBC020843
Gene Symbol HAVCR2
Gene ID (NCBI) 84868
RRIDAB_10734330
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8TDQ0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TIM3, also known as HAVCR2, is a member of the recently discovered T cell Ig and mucin domain-containing molecule superfamily. TIM3 is a negative regulatory molecule that is important for T cell tolerance and has a crucial role in autoimmunity and T cell exhaustion during chronic viral infection (PMID: 23180819). TIM3 is expressed by T-helper type 1 (Th1) cells, macrophage, monocyte, dendritic cells, CD8+ T cell and other lymphocyte subsets. Galectin-9 is a ligand for TIM3. TIM3-galectin-9 pathway negatively regulates T helper type 1 immunity (PMID: 16286920). TIM3 is a 280-aa membrane protein with a calculated molecular weight of 33 kDa, the higher molecular weights between 50 and 70 kDa probably represent glycosylated TIM3 (PMID: 20107545; 17069754; 11725301)

实验方案

Product Specific Protocols
WB protocol for TIM3 antibody 11872-1-APDownload protocol
FC protocol for TIM3 antibody 11872-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Front Mol Neurosci

Galectin-9/Tim-3 pathway mediates dopaminergic neurodegeneration in MPTP-induced mouse model of Parkinson’s disease

Authors - Qinyu Peng
humanWB

Front Cell Infect Microbiol

The Role of Tim-3 on dNK Cells Dysfunction During Abnormal Pregnancy With Toxoplasma gondii Infection.

Authors - Teng Li
humanIHC

CNS Neurosci Ther

PTX3 mediates the infiltration, migration, and inflammation-resolving-polarization of macrophages in glioblastoma.

Authors - Hao Zhang

Int J Oncol

Role of downregulation of galectin-9 in the tumorigenesis of gastric cancer.

Authors - Jiaxing Yang
mouseWB

Iran J Immunol

The Immunomodulatory Effects and Mechanisms of Tim-3 Action in the Early Stage of Mice with Severe Acute Pancreatitis.

Authors - Min Lin
humanWB

Immun Inflamm Dis

Galectin-9 and myeloid-derived suppressor cell as prognostic indicators for chronic lymphocytic leukemia

Authors - Xierenguli Alimu