验证数据展示
经过测试的应用
| Positive WB detected in | K-562 cells, DU 145 cells, mouse testis tissue, mouse thymus tissue, PC-3 cells |
| Positive IP detected in | mouse testis tissue |
| Positive IHC detected in | mouse thymus tissue, mouse testis tissue, rat testis tissue, rat thymus tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
| Positive FC (Intra) detected in | HepG2 cells |
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11955-1-AP targets HELLS in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description |
| Cited Applications | WB, IF |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Immunogen | HELLS fusion protein Ag2558 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | helicase, lymphoid-specific |
| Synonyms | Nbla10143, Lymphoid-specific helicase, Lymphoid specific helicase, LSH, helicase, lymphoid |
| Calculated Molecular Weight | 348 aa, 40 kDa, 97 kDa |
| Observed Molecular Weight | 97 kDa |
| GenBank Accession Number | BC031004 |
| Gene Symbol | HELLS |
| Gene ID (NCBI) | 3070 |
| RRID | AB_2117529 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NRZ9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Lymphoid-specific helicase(HELLS) is a member of the SNF2 family of chromatin remodeling proteins that is required for development and survival. It's required for de novo or maintenance DNA methylation, which mediated the silencing of the imprinted CDKN1C gene. Also it involved in formation and organization of heterochromatin that regulate transcription and mitosis.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for HELLS antibody 11955-1-AP | Download protocol |
| IHC protocol for HELLS antibody 11955-1-AP | Download protocol |
| IF protocol for HELLS antibody 11955-1-AP | Download protocol |
| IP protocol for HELLS antibody 11955-1-AP | Download protocol |
| FC protocol for HELLS antibody 11955-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun Germinal center output is sustained by HELLS-dependent DNA-methylation-maintenance in B cells | ||
Nucleic Acids Res The C-terminal 4CXXC-type zinc finger domain of CDCA7 recognizes hemimethylated DNA and modulates activities of chromatin remodeling enzyme HELLS | ||
Sci Adv CDCA7-associated global aberrant DNA hypomethylation translates to localized, tissue-specific transcriptional responses | ||
Genome Biol Lsh regulates LTR retrotransposon repression independently of Dnmt3b function. | ||
mSystems Adenovirus Remodeling of the Host Proteome and Host Factors Associated with Viral Genomes | ||
Hum Mol Genet Converging disease genes in ICF syndrome: ZBTB24 controls expression of CDCA7 in mammals. |
