验证数据展示
经过测试的应用
| Positive WB detected in | mouse testis tissue, A549 cells, NCI-H1299 cells, rat testis tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:3000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
19929-1-AP targets HEY1 in WB, IHC, IF, IP, CoIP, chIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP, CoIP, chIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | HEY1 fusion protein Ag13906 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | hairy/enhancer-of-split related with YRPW motif 1 |
| 别名 | HESR 1, HERP2, CHF-2, CHF2, CHF 2 |
| 计算分子量 | 304 aa, 33 kDa |
| 观测分子量 | 32-34 kDa |
| GenBank蛋白编号 | BC001873 |
| 基因名称 | HEY1 |
| Gene ID (NCBI) | 23462 |
| RRID | AB_10646438 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9Y5J3 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Hairy/enhancer of split-related proteins are basic helix-loop-helix (bHLH) transcription factors implicated in cell fate decision and boundary formation. And HEY1 is one of such protein. It is the direct downstream effector of Notch signaling which may be required for cardiovascular development. It preferentially bind to the canonical E box sequence 5'-CACGTG-3' and acts as a transcriptional repressor. HEY1 is a 33kDa protein and forms dimer by self-associating.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for HEY1 antibody 19929-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Metab Nicotinamide metabolism face-off between macrophages and fibroblasts manipulates the microenvironment in gastric cancer | ||
Protein Cell Microbiota enterotoxigenic Bacteroides fragilis-secreted BFT-1 promotes breast cancer cell stemness and chemoresistance through its functional receptor NOD1 | ||
Theranostics Nicotinamide mononucleotide enhances fracture healing by promoting skeletal stem cell proliferation | ||
J Exp Clin Cancer Res ACTN1 promotes HNSCC tumorigenesis and cisplatin resistance by enhancing MYH9-dependent degradation of GSK-3β and integrin β1-mediated phosphorylation of FAK | ||
Dev Cell Regulation of reverse electron transfer at mitochondrial complex I by unconventional Notch action in cancer stem cells. | ||
Nat Commun Cathepsin K-mediated notch1 activation contributes to neovascularization in response to hypoxia. |
