验证数据展示
经过测试的应用
| Positive WB detected in | Cobalt Chloride treated HeLa cells, HeLa cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human thyroid cancer tissue, human heart tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | Cobalt Chloride treated HeLa cells, Cobalt Chloride treated HepG2 cells |
Samples need to be treated with hypoxia stimulation.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
20960-1-AP targets HIF-1 alpha in WB, IHC, IF/ICC, IP, CoIP, chIP, RIP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP, CoIP, chIP, RIP |
| 经测试反应性 | human |
| 文献引用反应性 | human, pig, chicken, goat |
| 免疫原 | HIF-1 alpha fusion protein Ag15198 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor) |
| 别名 | HIF1A, HIF 1α, HIF 1 alpha, Class E basic helix-loop-helix protein 78, Basic-helix-loop-helix-PAS protein MOP1 |
| 计算分子量 | 826 aa, 93 kDa |
| 观测分子量 | 120 kDa |
| GenBank蛋白编号 | BC012527 |
| 基因名称 | HIF1A |
| Gene ID (NCBI) | 3091 |
| RRID | AB_10732601 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q16665 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
HIF1a, the major regulator of the cellular responses to hypoxia, consists of an oxygen-sensitive subunit, HIF1 alpha (HIF1A), and an oxygen-insensitive subunit, HIF1 beta (arylhydrocarbon receptor nuclear transporter [ARNT]). Under normal oxygen conditions, HIF1a is continuously produced and destroyed, in a process involving hydroxylation, interaction with von Hippel-Lindau (VHL) protein, polyubiquitylation and subsequent proteasomal degradation. Under hypoxic conditions, hydroxylation is impaired and HIF1a is stabilized. HIF1a localizes in cytoplasm in normoxia, but it can translocate into nuclear in response to hypoxia. The calculated molecular weight of HIF1a is 93 kDa, but the modified protein HIF1a is about 110-120kDa (PMID: 11698256, .PMID: 7539918). .
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for HIF-1 alpha antibody 20960-1-AP | Download protocol |
| IHC protocol for HIF-1 alpha antibody 20960-1-AP | Download protocol |
| IF protocol for HIF-1 alpha antibody 20960-1-AP | Download protocol |
| IP protocol for HIF-1 alpha antibody 20960-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Gastroenterology Pancreatic acinar cells-derived sphingosine-1-phosphate contributes to fibrosis of chronic pancreatitis via inducing autophagy and activation of pancreatic stellate cells | ||
Cancer Cell Cancer cell autophagy, reprogrammed macrophages, and remodeled vasculature in glioblastoma triggers tumor immunity | ||
Nat Cell Biol A single-cell transcriptomic landscape of the lungs of patients with COVID-19. | ||
ACS Nano Augmenting Intracellular Cargo Delivery of Extracellular Vesicles in Hypoxic Tissues through Inhibiting Hypoxia-Induced Endocytic Recycling | ||
Adv Sci (Weinh) Inflammatory Fibroblast-Like Synoviocyte-Derived Exosomes Aggravate Osteoarthritis via Enhancing Macrophage Glycolysis |
