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HK3 Monoclonal antibody

HK3 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

3H3C7

Cat no : 67803-1-Ig

Print datasheet

Synonyms

Hexokinase-C, Hexokinase-3, Hexokinase type III, hexokinase 3 (white cell), Hexokinase 3

验证数据展示

  • WB analysis of human saliva using 67803-1-Ig

    WB analysis of human saliva using 67803-1-Ig

    human saliva were subjected to SDS PAGE followed by western blot with 67803-1-Ig (HK3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of rat spleen using 67803-1-Ig

    WB analysis of rat spleen using 67803-1-Ig

    rat spleen tissue were subjected to SDS PAGE followed by western blot with 67803-1-Ig (HK3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of Raji using 67803-1-Ig

    WB analysis of Raji using 67803-1-Ig

    Raji cells were subjected to SDS PAGE followed by western blot with 67803-1-Ig (HK3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of Ramos using 67803-1-Ig

    WB analysis of Ramos using 67803-1-Ig

    Ramos cells were subjected to SDS PAGE followed by western blot with 67803-1-Ig (HK3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of RAW 264.7 using 67803-1-Ig

    WB analysis of RAW 264.7 using 67803-1-Ig

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 67803-1-Ig (HK3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 67803-1-Ig

    IHC staining of human lung cancer using 67803-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67803-1-Ig (HK3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human lung cancer using 67803-1-Ig

    IHC staining of human lung cancer using 67803-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67803-1-Ig (HK3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inRaji cells, Ramos cells, RAW 264.7 cells, human saliva, rat spleen tissue
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

67803-1-Ig targets HK3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat, pig
Cited Reactivityhuman, mouse
Immunogen HK3 fusion protein Ag30798 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name hexokinase 3 (white cell)
Synonyms Hexokinase-C, Hexokinase-3, Hexokinase type III, hexokinase 3 (white cell), Hexokinase 3
Calculated Molecular Weight 923 aa, 99 kDa
Observed Molecular Weight99 kDa
GenBank Accession NumberBC028129
Gene Symbol HK3
Gene ID (NCBI) 3101
RRIDAB_2918566
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP52790
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Hexokinases phosphorylate glucose to produce glucose-6-phosphate (G6P), the first step in most glucose metabolism pathways. Hexokinase 3 also known as HK3, similar to hexokinases 1 and 2, this allosteric enzyme is inhibited by its product glucose-6-phosphate.

实验方案

Product Specific Protocols
WB protocol for HK3 antibody 67803-1-IgDownload protocol
IHC protocol for HK3 antibody 67803-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,mouseWB

Biochem Biophys Res Commun

Identification of HK3 as a promising immunomodulatory and prognostic target in sepsis-induced acute lung injury

Authors - Mingyu Zhu
View Article
humanWB

Biochem Biophys Res Commun

HK3: A potential prognostic biomarker with metastasis inhibition capabilities in hepatocellular carcinoma

Authors - Yexiang Du
View Article