验证数据展示
经过测试的应用
Positive IF-P detected in | mouse spleen tissue, human liver tissue |
Positive FC (Intra) detected in | HeLa cells |
推荐稀释比
Application | Dilution |
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Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL594-66743 targets HO-1/HMOX1 in IF-P, FC (Intra) applications and shows reactivity with human, mouse, rat, pig samples.
Tested Applications | IF-P, FC (Intra) Application Description |
Tested Reactivity | human, mouse, rat, pig |
Immunogen | HO-1/HMOX1 fusion protein Ag21296 种属同源性预测 |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Full Name | heme oxygenase (decycling) 1 |
Synonyms | HMOX1, HO-1, Heme oxygenase 1 soluble form, Heme oxygenase 1, heme oxygenase (decycling) 1 |
Calculated Molecular Weight | 33 kDa |
Observed Molecular Weight | 33 kDa |
GenBank Accession Number | BC001491 |
Gene Symbol | HO-1 |
Gene ID (NCBI) | 3162 |
RRID | AB_2883623 |
Conjugate | CoraLite®594 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 588 nm / 604 nm |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P09601 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Heme oxygenase (HMOX1) catalyzes the first and rate-limiting step in the degradation of heme to yield equimolar quantities of biliverdin Ixa, carbon monoxide (CO), and iron. It has 3 isoforms: HO-1 is highly inducible, whereas HO-2 and HO-3 are constitutively expressed (PMID:10194478). Heme oxygenase-1 (HO-1) is expressed in many tissues and vascular smooth muscle cells, and endothelial cells (PMID:15451051) and has been identified as an important endogenous protective factor induced in many cell types by various stimulants, such as hemolysis, infiammatory cytokines,oxidative stress, heat shock, heavy metals, and endotoxin (PMID: 11522663). And the full-length HO-1 is very unstable and susceptible to truncation that generates an inactive, soluble form (28 kDa) (James R. Reed, Pharmacology, 535-568).
实验方案
Product Specific Protocols | |
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IF protocol for CL594 HO-1/HMOX1 antibody CL594-66743 | Download protocol |
FC protocol for CL594 HO-1/HMOX1 antibody CL594-66743 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |