验证数据展示
经过测试的应用
| Positive WB detected in | human placenta tissue, pig adrenal gland tissue, human adrenal gland tissue |
| Positive IF/ICC detected in | A549 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
67572-1-Ig targets HSD3B2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, pig samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, pig |
| 文献引用反应性 | human, mouse |
| 免疫原 | HSD3B2 fusion protein Ag4291 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 2 |
| 别名 | 3-beta-HSD II, 3-beta-HSD adrenal and gonadal type, 3b HSD, 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type II, 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 2 |
| 计算分子量 | 42 kDa |
| 观测分子量 | 42 kDa |
| GenBank蛋白编号 | BC038419 |
| 基因名称 | HSD3B2 |
| Gene ID (NCBI) | 3284 |
| RRID | AB_2882785 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P26439 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
HSD3B2(3-beta-hydroxy-Delta(5)-steroid dehydrogenase) belongs to the 3-beta-HSD family and catalyzes the oxidation and isomerization of delta-5-3-beta-hydroxysteroid precursors into delta-4-ketosteroids, thus leading to the formation of all classes of steroid hormones.HSD3B2 is expressed almost exclusively in adrenals and gonads, whereas HSD3B1 is expressed predominantly in placenta and skin(PMID:1682237). Defects in HSD3B2 are the cause of adrenal hyperplasia type 2 (AH2).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for HSD3B2 antibody 67572-1-Ig | Download protocol |
| IF protocol for HSD3B2 antibody 67572-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Part Fibre Toxicol Chronic exposure to polystyrene microplastics induced male reproductive toxicity and decreased testosterone levels via the LH-mediated LHR/cAMP/PKA/StAR pathway. | ||
Ecotoxicol Environ Saf Biochemical mechanisms of tributyltin chloride-induced cell toxicity in Sertoli cells | ||
Birth Defects Res Bisphenol A decreases progesterone synthesis in human ovarian granulosa cells. | ||
Gynecol Endocrinol N6-methyladenosine demethylase FTO related to hyperandrogenism in PCOS via AKT pathway | ||
Pathol Res Pract Disorganized adrenocortical zonational structure in COVID-19 patients: Implications of critical illness duration | ||
Metabolism Increased adrenal steroidogenesis and suppressed corticosteroid responsiveness in critical COVID-19 |
