Human BIRC5 ELISA Kit

Catalog number: KE00043

规格

单价

96 T

¥2600

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Overview


Product name:
Human BIRC5 ELISA Kit

Tests:
1 X 96 well plate

Sample type:
serum, plasma, cell culture supernatant, urine

Assay type:
Sandwich

Sensitivity:
35 pg/mL

Range:
62.5 pg/mL-4000 pg/mL

Reacted Species:
Human

Tested applications:
Sandwich ELISA

Recovery:
Sample TypeAverageRange
Plasma 97% 82-125%
Cell culture supernatant 109% 91-125%
Urine 100% 80-119%

Product overview:
KE00043 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The BIRC5 ELISA kit is to be used to detect and quantify protein levels of endogenous BIRC5. The assay recognizes human BIRC5. A polyclonal antibody specific for BIRC5 has been pre-coated onto the microwells. The BIRC5 protein in samples is captured by the coated antibody after incubation. Following extensive washing, a monoclonal antibody specific for BIRC5 is added to detect the captured BIRC5 protein. For signal development, horseradish peroxidase (HRP)-conjugated Anti-mouse antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm.

Properties


Storage Instructions:
Store standard and Plate at -20° the rests store at 4°.Refer to the protocol for further storage instructions

Synonyms:
SURVIVIN, API4, IAP4, EPR 1
Background

Survivin, also called BIRC5, is a unique member of the inhibitor of apoptosis (IAP) protein family. Survivin is a 16 kDa anti-apoptotic protein highly expressed during fetal development and cancer cell malignancy, but is completely absent in terminally differentiated cells. The differential expression of survivin in cancer versus normal tissues makes it a useful tool in cancer diagnosis and a promising therapeutic target. Survivin expression is also highly regulated by the cell cycle and is only expressed in the G2-M phase. It is known that survivin localizes to the mitotic spindle by interaction with tubulin during mitosis and may play a contributing role in regulating mitosis. Disruption of survivin-microtubule interactions results in loss of survivin's anti-apoptosis function and increased caspase-3 activity, a mechanism involved in cell death, during mitosis. It also is a direct target gene of the Wnt pathway and is upregulated by beta-catenin.


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