Human CCL4 ELISA Kit0 Publications

Catalog number: KE00062


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Product name:
Human CCL4 ELISA Kit

1 X 96 well plate

Sample type:
Cell culture supernatant

Assay type:

1 pg/mL

7.8-500 pg/mL

Reacted Species:

Tested applications:
Sandwich ELISA

Sample TypeAverageRange
Cell culture supernatant 101% 92%-113%

Samplenmean (pg/mL)SDCV%
1 20 ? ug/ml ? ?%
2 20 ? ug/ml ? ?%
3 20 ? ug/ml ? ?%

Samplenmean (pg/mL)SDCV%
1 20 ? ug/ml ? ?%
2 20 ? ug/ml ? ?%
3 20 ? ug/ml ? ?%

Product overview:
KE00062 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The CCL4 ELISA kit is to be used to detect and quantify protein levels of endogenous CCL4. The assay recognizes human CCL4. A polyclonal antibody specific for CCL4 has been pre-coated onto the microwells. The CCL4 protein in samples is captured by the coated antibody after incubation. Following extensive washing, a monoclonal antibody specific for CCL4 is added to detect the captured CCL4 protein. For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm.


Storage Instructions:
All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.

ACT2, C C motif chemokine 4, CCL4, chemokine (C C motif) ligand 4, HC21, LAG 1, LAG1, MIP 1 beta, MIP1B, MIP1B1, PAT 744, SCYA2, SCYA4, Small inducible cytokine A4

CCL4, also known as Macrophage inflammatory protein-1β (MIP-1β), is a CC chemokine with specificity for CCR5 receptors, and is one of the major HIV-suppressive factors produced by CD8+ T-cells. MIP-1β is an acidic protein composed of 69 amino acids that is produced by many cells, particularly macrophages, dendritic cells, and lymphocytes. MIP-1β is responsible for the activation of PMN and is involved in acute neutrophilic inflammation. Recombinant MIP-1β induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV).

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