Human CD13 ELISA Kit

Catalog number: KE00083

规格

单价

96 T

¥2600

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Overview


Product name:
Human CD13 ELISA Kit

Tests:
1 X 96 well plate

Sample type:
Serum, Plasma, Cell culture supernatant

Assay type:
Sandwich

Sensitivity:
0.06 ng/mL

Range:
0.313-20 ng/mL

Reacted Species:
Human

Tested applications:
Sandwich ELISA

Recovery:
Sample TypeAverageRange
Plasma 91% 79%-105%
Cell culture supernatant 106% 83%-127%

Product overview:
KE00083 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The CD13 ELISA kit is to be used to detect and quantify protein levels of endogenous CD13. The assay recognizes human CD13. A polyclonal antibody specific for CD13 has been pre-coated onto the microwells. The CD13 protein in samples is captured by the coated antibody after incubation. Following extensive washing, a monoclonal antibody specific for CD13 is added to detect the captured CD13 protein. For signal development, horseradish peroxidase (HRP)-conjugated Anti-mouse antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm.

Properties


Storage Instructions:
All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.

Synonyms:
Alanyl aminopeptidase, Aminopeptidase M, Aminopeptidase N, ANPEP, AP M, AP N, APN, CD13, gp150, hAPN, LAP1, Microsomal aminopeptidase, p150, PEPN
Background

CD13 is also named as APN, ANPEP(aminopeptidase N), PEPN and belongs to the peptidase M1 family. It is a 150 kDa cell surface glycoprotein originally identified on subsets of normal and malignant human myeloid cells CD13 reduced ROS-induced DNA damage after genotoxic chemo/radiation stress and protected cells from apoptosis. CD13 is identified as a protein marker correlating with an enrichment of dormant, slow-growing malignant stem cells, with most CD13+ cells resting in the G1/G0 phase of the cell cycle.


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