Human FGF12 ELISA Kit0 Publications

Catalog number: KE00036


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Product name:
Human FGF12 ELISA Kit

1 X 96 well plate

Sample type:
Plasma, Serum, Cell culture supernatant

Assay type:

0.015 ng/mL

0.5-32 ng/mL

Reacted Species:

Tested applications:
Sandwich ELISA

Sample TypeAverageRange
Plasma 91% 72%-125%
Cell culture supernatant 102% 82%-120%

Samplenmean (ng/mL)SDCV%
1 20 ? ug/ml ? ?%
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3 20 ? ug/ml ? ?%

Samplenmean (ng/mL)SDCV%
1 20 ? ug/ml ? ?%
2 20 ? ug/ml ? ?%
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Product overview:
KE00036 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The FGF12 ELISA kit is to be used to detect and quantify protein levels of endogenous FGF12. The assay recognizes human FGF12. A polyclonal antibody specific for FGF12 has been pre-coated onto the microwells. The FGF12 protein in samples is captured by the coated antibody after incubation. Following extensive washing, a monoclonal antibody specific for FGF12 is added to detect the captured FGF12 protein. For signal development, horseradish peroxidase (HRP)-conjugated Anti-mouse antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm.


Storage Instructions:
All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.

FGF 12, FGF12, FGF12B, FHF 1, FHF1, fibroblast growth factor 12, Myocyte activating factor

FGF12 is a member of the fibroblast growth factor (FGF) family. FGF family members play important roles in embryogenesis, angiogenesis, and wound repair. FGF12 lacks the N-terminal signal sequence present in most of the FGF family members, but it contains clusters of basic residues that have been demonstrated to act as a nuclear localization signal. When transfected into mammalian cells, this protein accumulated in the nucleus, but was not secreted. FGF12 plays an intracellular role in the inhibition of radiation-induced apoptosis. FGF12 is expressed abundantly in developing and adult nervous systems; therefore, FGF12 was thought to be related to nervous system development and function.

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