Human GMFG ELISA Kit

Catalog number: KE00087

规格

单价

96 T

¥2600

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Overview


Product name:
Human GMFG ELISA Kit

Tests:
1 X 96 well plate

Sample type:
Serum, Plasma, Cell culture supernatant

Assay type:
Sandwich

Sensitivity:
13 pg/mL

Range:
39-2500 pg/mL

Reacted Species:
Human

Tested applications:
Sandwich ELISA

Recovery:
Sample TypeAverageRange
Plasma 96% 76%-128%
Cell culture supernatant 90% 78%-104%

Product overview:
KE00087 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The GMFG ELISA kit is to be used to detect and quantify protein levels of endogenous GMFG. The assay recognizes human GMFG. A polyclonal antibody specific for GMFG has been pre-coated onto the microwells. The GMFG protein in samples is captured by the coated antibody after incubation. Following extensive washing, a monoclonal antibody specific for GMFG is added to detect the captured GMFG protein. For signal development, horseradish peroxidase (HRP)-conjugated Anti-mouse antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm.

Properties


Storage Instructions:
All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.

Synonyms:
Glia maturation factor gamma, glia maturation factor, gamma, GMF GAMMA, GMFG
Background

GMFG (glia maturation factor-γ), is a highly conserved brain-specific protein that belongs to the GMF subfamily of the larger Actin-binding protein ADF family. GMFG is preferentially expressed in blood vessel cells and immune cells and its ectopic expression enhances cellular functions such as tube-formation and migration in vitro. GMFG mediates neutrophil and T-lymphocyte migration via regulation of actin cytoskeletal reorganization. GMFG also acts as an intracellular regulator of stress-activated signal transduction by demonstrating activation of p38 MAP kinase and transcription factor NF-kB in astrocytes.


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