Human IFN-gamma ELISA Kit

Catalog number: KE00063

规格

单价

96 T

¥2600

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Overview


Product name:
Human IFN-gamma ELISA Kit

Tests:
1 X 96 well plate

Sample type:
Serum, Plasma, Cell culture supernatant

Assay type:
Sandwich

Sensitivity:
1 pg/mL

Range:
15.6-1000 pg/mL

Reacted Species:
Human

Tested applications:
Sandwich ELISA

Recovery:
Sample TypeAverageRange
Plasma 95% 86%-102%
Cell culture supernatant 111% 84%-121%

Product overview:
KE00063 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The IFN-gamma ELISA kit is to be used to detect and quantify protein levels of endogenous IFN-gamma. The assay recognizes human IFN-gamma. A monoclonal antibody specific for IFN-gamma has been pre-coated onto the microwells. The IFN-gamma protein in samples is captured by the coated antibody after incubation. Following extensive washing, a polyclonal antibody of biotinylated specific for IFN-gamma is added to detect the captured IFN-gamma protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm.

Properties


Storage Instructions:
Store standard and Plate at -20° the rests store at 4°.Refer to the protocol for further storage instructions

Synonyms:
Ifg, IFN g, Ifn Gamma, Ifng, interferon gamma
Background

Interferon gamma (IFNG) is a soluble cytokine that is the only member of the type II class of interferons. It is secreted by Th1 cells, cytotoxic T cells and NK cells. The cytokine is associated with antiviral, immunoregulatory and anti-tumor properties and is a potent activator of macrophages. It plays crucial roles in pathogen clearance. Aberrant IFNG expression is associated with a number of autoinflammatory and autoimmune diseases. It has been identified in many studies as a biomarker for pleural tuberculosis (TB). Mutations in this gene are associated with aplastic anemia.


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