Human IL31 ELISA Kit0 Publications

Catalog number: KE00086



96 T


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Product name:
Human IL31 ELISA Kit

1 X 96 well plate

Sample type:
Serum, Plasma, Cell culture supernatant

Assay type:

7.4 pg/mL

31.25 - 2000 pg/mL

Reacted Species:

Tested applications:
Sandwich ELISA

Sample TypeAverageRange
Cell culture supernatant 91% 83%-113%
Human serum 91% 74%-117%

Samplenmean (pg/mL)SDCV%
1 20 41.0 1.7 4.2
2 20 431.6 10.7 2.5
3 20 1,731.5 52.7 3.0

Samplenmean (pg/mL)SDCV%
1 24 37.4 3.3 8.9
2 24 415.9 15.1 3.6
3 24 1,726.2 74.8 4.3

Product overview:
KE00086 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Human IL31 ELISA kit is to be used to detect and quantify protein levels of endogenous human IL31. The assay recognizes human IL31. A monoclonal antibody specific for human IL31 has been pre-coated onto the microwells. The human IL31 protein in samples is captured by the coated antibody after incubation. Following extensive washing, a monoclonal antibody of biotinylated specific for human IL31 is added to detect the captured human IL31 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm.


Storage Instructions:
All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.

IL 31, IL31, IL-31, interleukin 31

Interleukin-31 (IL-31) is a newly discovered four-helix bundle cytokine and expressed by several types of cells under both normal and disease state, such as activated CD4+T cells, mast cells, monocytes, macrophages, immature and mature monocyte-derived dendritic cells and so on. IL-31 signals through a heterodimeric receptor complex consisting of the IL31 receptor alpha (IL31Rα) and the oncostatin M receptor (OSMR). IL-31 plays a predominant function in the mediation of inflammatory and lymphoma-associated itch. Increased IL-31 expression has been detected in inflamed tissues from patients with atopic dermatitis, bowel diseases, allergic asthma and rhinitis.

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