Human Leptin ELISA Kit0 Publications

Catalog number: KE00095



96 T


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Product name:
Human Leptin ELISA Kit

1 X 96 well plate

Sample type:
Serum, Plasma, Cell culture supernatant

Assay type:

2.0 pg/mL

15.6 - 500 pg/mL

Reacted Species:

Tested applications:
Sandwich ELISA

Sample TypeAverageRange
Cell culture supernatant 98% 80%-117%
Human plasma 94% 82%-113%

Samplenmean (pg/mL)SDCV%
1 20 79.7 2.8 3.5
2 20 182.7 5.8 3.2
3 20 461.7 20.9 4.5

Samplenmean (pg/mL)SDCV%
1 24 84.3 1.9 2.2
2 24 185.1 4.6 2.5
3 24 461.7 15.4 3.3

Product overview:
KE00095 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human leptin ELISA kit is to be used to detect and quantify protein levels of endogenous human . The assay recognizes human leptin. A monoclonal antibody specific for human leptin has been pre-coated onto the microwells. The human leptin protein in samples is captured by the coated antibody after incubation. Following extensive washing, a monoclonal antibody of biotinylated specific for human leptin is added to detect the captured human leptin protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm.


Storage Instructions:
All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.

LEP, leptin, OB, Obese protein, Obesity factor, OBS

Leptin is a 16 kDa adipocyte-derived hormone, and it has attracted interest in the field of obesity research due to its role in the regulation of energy balance. Leptin, secreted from adipose tissues in proportion to the fat store, acts in the hypothalamus to regulate feeding behavior. leptin has also been shown to play an essential role in tumorigenesis, tumor progression, and metastasis, due to its oncogenic, mitogenic, pro-inflammatory, and pro-angiogenic actions. Mutations in this gene and/or its regulatory regions cause severe obesity, and morbid obesity with hypogonadism. Leptin has also been linked to type 2 diabetes mellitus development.

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