Human MMP-13 ELISA Kit0 Publications

Catalog number: KE00078



96 T


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Product name:
Human MMP-13 ELISA Kit

1 X 96 well plate

Sample type:
Serum, Plasma, Cell culture supernatant

Assay type:

1.2 pg/mL

93.8-6000 pg/ml

Reacted Species:

Tested applications:
Sandwich ELISA

Sample TypeAverageRange
Cell culture supernatant 86% 79%-92%
Human plasma 90% 82%-100%

Samplenmean (pg/mL)SDCV%
1 20 928.6 69.5 7.5
2 20 1,630.0 101.7 6.2
3 20 3,808.3 227.1 6.0

Samplenmean (pg/mL)SDCV%
1 24 1,002.6 78.1 7.8
2 24 1,833.0 111.3 6.1
3 24 4,422.9 363.0 8.2

Product overview:
KE00078 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The MMP-13 ELISA kit is to be used to detect and quantify protein levels of endogenous MMP-13. The assay recognizes human MMP-13. A monoclonal antibody specific for MMP-13 has been pre-coated onto the microwells. The MMP-13 protein in samples is captured by the coated antibody after incubation. Following extensive washing, a polyclonal antibody of biotinylated specific for MMP-13 is added to detect the captured MMP-13 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm.


Storage Instructions:
All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.

CLG3, Collagenase 3, Matrix metalloproteinase 13, MMP 13, MMP13

MMP-13 (Matrix metalloproteinase 13), also known as collagenase-3, cleaves type I collagen and was previously thought to be the rodent analogue of human interstitial collagenase (MMP-1). MMP-3 is also known to activate several other MMPs such as MMP-7 and MMP-9. All the above make it a crucial factor in connective tissue remodeling. MMP-13 is found in hypertrophic and calcifying cartilage of mammalian growth plate. It can be detected 3 bands that the glycosylated pro MMP-13 (70 kDa), unglycosylated proform (55 kDa), and active MMP-13 enzyme (40kDa) by western blot.

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