Human PGA5 ELISA Kit0 Publications

Catalog number: KE00084



96 T


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Product name:
Human PGA5 ELISA Kit

1 X 96 well plate

Sample type:
Serum, Plasma, Cell culture supernatant

Assay type:

0.16 ng/mL

0.5-32 ng/mL

Reacted Species:

Tested applications:
Sandwich ELISA

Sample TypeAverageRange
Cell culture supernatant 104% 78%-127%
Human plasma 93% 75%-115%

Samplenmean (ng/mL)SDCV%
1 20 24.36 1.33 5.5
2 20 6.43 0.33 5.2
3 20 0.65 0.06 8.9

Samplenmean (ng/mL)SDCV%
1 24 23.79 1.86 7.8
2 24 5.78 0.49 8.5
3 24 0.48 0.04 9.0

Product overview:
KE00084 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The PGA5 ELISA kit is to be used to detect and quantify protein levels of endogenous PGA5. The assay recognizes human PGA5. A polyclonal antibody specific for PGA5 has been pre-coated onto the microwells. The PGA5 protein in samples is captured by the coated antibody after incubation. Following extensive washing, a monoclonal antibody specific for PGA5 is added to detect the captured PGA5 protein. For signal development, horseradish peroxidase (HRP)-conjugated Anti-mouse antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm.


Storage Instructions:
All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.

Pepsin A, PGA3, PGA4, PGA5

Pepsinogen I belongs to the peptidase A1 family. The gene endoces a 388 amino acid protein with a 15 amino acid signal peptide and 46 amino acid propeptide. Pepsinogen I is secreted only by the chief cells of the corpus, and its serum level decreases with increasing grade of the corpus atrophy (loss of oxyntic glands). Low serum levels of pepsinogen I are indicative of chronic atrophy, a risk factor for gastric cancer.

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