Human PGA5 ELISA Kit

Catalog number: KE00084

规格

单价

96 T

¥2600

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Overview


Product name:
Human PGA5 ELISA Kit

Tests:
1 X 96 well plate

Sample type:
Serum, Plasma, Cell culture supernatant

Assay type:
Sandwich

Sensitivity:
0.16 ng/mL

Range:
0.5-32 ng/mL

Reacted Species:
Human

Tested applications:
Sandwich ELISA

Recovery:
Sample TypeAverageRange
Plasma 93% 75%-115%
Cell culture supernatant 104% 78%-127%

Product overview:
KE00084 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The PGA5 ELISA kit is to be used to detect and quantify protein levels of endogenous PGA5. The assay recognizes human PGA5. A polyclonal antibody specific for PGA5 has been pre-coated onto the microwells. The PGA5 protein in samples is captured by the coated antibody after incubation. Following extensive washing, a monoclonal antibody specific for PGA5 is added to detect the captured PGA5 protein. For signal development, horseradish peroxidase (HRP)-conjugated Anti-mouse antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm.

Properties


Storage Instructions:
Store standard and Plate at -20° the rests store at 4°.Refer to the protocol for further storage instructions

Synonyms:
Pepsin A, pepsinogen 5, group I (pepsinogen A), Pepsinogen I, PGA3, PGA4
Background

Pepsinogen I belongs to the peptidase A1 family. The gene endoces a 388 amino acid protein with a 15 amino acid signal peptide and 46 amino acid propeptide. Pepsinogen I is secreted only by the chief cells of the corpus, and its serum level decreases with increasing grade of the corpus atrophy (loss of oxyntic glands). Low serum levels of pepsinogen I are indicative of chronic atrophy, a risk factor for gastric cancer.


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