Human PGA5 ELISA Kit

Sensitivity

0.16 ng/mL

Range

0.5-32 ng/mL

Reactivity

Human

Cat No : KE00084

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Synonyms

Pepsin A, PGA3, PGA4, PGA5



Product Information

KE00084 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The PGA5 ELISA kit is to be used to detect and quantify protein levels of endogenous PGA5. The assay recognizes human PGA5. An antibody specific for PGA5 has been pre-coated onto the microwells. The PGA5 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody specific for PGA5 is added to detect the captured PGA5 protein. For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.

Product name Human PGA5 ELISA Kit
Tests 1 X 96 well plate
Sample type Serum, Plasma, Cell culture supernatants
Assay type Sandwich
Sensitivity 0.16 ng/mL
Range 0.5-32 ng/mL
Reactivity Human
Tested applications Sandwich ELISA
Gene ID (NCBI) 5222

Recovery

Sample TypeAverageRange
Human plasma93%75%-115%
Cell culture supernatants104%78%-127%

IntraAssay

Samplenmean ( ng/mL)SDCV%
12024.361.335.5
2206.430.335.2
3200.650.068.9

InterAssay

Samplenmean ( ng/mL)SDCV%
12423.791.867.8
2245.780.498.5
3240.480.049.0

Background Information

Pepsinogen I belongs to the peptidase A1 family. The gene endoces a 388 amino acid protein with a 15 amino acid signal peptide and 46 amino acid propeptide. Pepsinogen I is secreted only by the chief cells of the corpus, and its serum level decreases with increasing grade of the corpus atrophy (loss of oxyntic glands). Low serum levels of pepsinogen I are indicative of chronic atrophy, a risk factor for gastric cancer.

Properties

Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.
Synonyms Pepsin A, PGA3, PGA4, PGA5