Human RBP4 ELISA Kit0 Publications

Catalog number: KE00056



96 T


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Product name:
Human RBP4 ELISA Kit

1 X 96 well plate

Sample type:
Serum, Plasma, Cell culture supernatant, Urine, Saliva

Assay type:

2.3 pg/mL

31.25-1000 pg/mL

Reacted Species:

Tested applications:
Sandwich ELISA

Sample TypeAverageRange
Plasma 99% 88%-114%
Cell culture supernatant 100% 84%-118%
Urine 104% 93%-112%
Saliva 111% 94%-121%

Samplenmean (pg/mL)SDCV%
1 20 500.0 13.9 2.8
2 20 115.0 3.1 2.7
3 20 22.9 2.2 9.4

Samplenmean (pg/mL)SDCV%
1 20 572.0 42.3 7.4
2 20 115.6 8.1 7.0
3 20 33.0 3.2 9.9

Product overview:
KE00056 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The RBP4 ELISA kit is to be used to detect and quantify protein levels of endogenous RBP4. The assay recognizes human RBP4. A monoclonal antibody specific for RBP4 has been pre-coated onto the microwells. The RBP4 protein in samples is captured by the coated antibody after incubation. Following extensive washing, a polyclonal antibody of biotinylated specific for RBP4 is added to detect the captured RBP4 protein. For signal development, Sterptravidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm.


Storage Instructions:
All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.

Plasma retinol binding protein, PRBP, RBP, RBP4, Retinol binding protein 4

Retinol-binding protein 4 (RBP4; formerly called RBP) is a plasma transport protein for vitamin A (retinol). Synthesized primarily by hepatocytes and adipocytes, RBP4 is secreted into the circulation as a retinol-RBP complex and functions to transport retinol to peripheral tissues. In plasma the RBP4-retinol complex is bound to transthyretin (TRR) to prevent kidney filtration. The C-terminally processed forms of RBP4, which do not bind TTR, are normally excreted into the urine but accumulate in the serum during renal failure. Urinary RBP4 has been reported as marker for glomerular disease. RBP4 also was identified as an adipokine that elevated in some insulin-resistant states. Measurement of serum RBP4 could be used to assess the risk of insulin resistance, type 2 diabetes, obesity, and cardiovascular disease.

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