Human GRP ELISA Kit

Catalog number: KE00070

规格

单价

96 T

¥2600

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Overview


Product name:
Human GRP ELISA Kit

Tests:
1 X 96 well plate

Sample type:
Serum, Plasma, Cell culture supernatant

Assay type:
Sandwich

Sensitivity:
0.03 ng/mL

Range:
0.313 ng/mL - 20 ng/mL

Reacted Species:
Human

Tested applications:
Sandwich ELISA

Recovery:
Sample TypeAverageRange
Plasma 108% 86%-128%
Cell culture supernatant 91% 77%-103%

Product overview:
KE00070 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The GRP ELISA kit is to be used to detect and quantify protein levels of endogenous GRP. The assay recognizes human GRP. A polyclonal antibody specific for GRP has been pre-coated onto the microwells. The GRP protein in samples is captured by the coated antibody after incubation. Following extensive washing, a monoclonal antibody specific for GRP is added to detect the captured GRP protein. For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm.

Properties


Storage Instructions:
Store standard and Plate at -20° the rests store at 4°.Refer to the protocol for further storage instructions

Synonyms:
BN, gastrin releasing peptide, GRP, GRP 10, preproGRP, proGRP
Background

Gastrin-releasing peptide, also known as GRP, is normally formed by mucosal cells in the gastric antrum and by the D cells of the pancreatic islets, and its main function is to stimulate secretion of HCl by the gastric mucosa. HCl, in turn, inhibits gastrin formation. Its 148-amino acid preproprotein, following cleavage of a signal peptide, is further processed to produce either the 27-amino acid gastrin-releasing peptide or the 10-amino acid neuromedin C. These smaller peptides regulate numerous functions of the gastrointestinal and central nervous systems, including release of gastrointestinal hormones, smooth muscle cell contraction, and epithelial cell proliferation.These peptides are also likely to play a role in human cancers of the lung, colon, stomach, pancreas, breast, and prostate.


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