Human SELP ELISA Kit

Catalog number: KE00064

规格

单价

96 T

¥2600

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Overview


Product name:
Human SELP ELISA Kit

Tests:
1 X 96 well plate

Sample type:
Serum, Plasma, Cell culture supernatant

Assay type:
Sandwich

Sensitivity:
0.025 ng/mL

Range:
0.25 ng/mL - 16 ng/mL

Reacted Species:
Human

Tested applications:
Sandwich ELISA

Recovery:
Sample TypeAverageRange
Plasma 93% 78%-110%
Cell culture supernatant 92% 83%-113%

Product overview:
KE00064 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The SELP ELISA kit is to be used to detect and quantify protein levels of endogenous SELP. The assay recognizes human SELP. A polyclonal antibody specific for SELP has been pre-coated onto the microwells. The SELP protein in samples is captured by the coated antibody after incubation. Following extensive washing, a monoclonal antibody specific for SELP is added to detect the captured SELP protein. For signal development, horseradish peroxidase (HRP)-conjugated Anti-mouse antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm.

Properties


Storage Instructions:
Store standard and Plate at -20° the rests store at 4°.Refer to the protocol for further storage instructions

Synonyms:
CD62, CD62P, FLJ45155, GMP 140, GMP140, GMRP, P selectin
Background

SELP, also named as P-selectin, GMP-140 or CD62P, is a transmembrane glycoprotein that mediates the interaction of activated endothelial cells or platelets with leukocytes. It is an adhesion molecule involved in the pathogenesis of inflammation, thrombosis, and oncogenesis. P-selectin is stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. Upon cell activation by agonists, P-selectin is transported rapidly to the cell surface. Soluble P-selectin is produced by platelets and endothelial cells and circulates in normal plasma.


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