Human ST2 ELISA Kit

Catalog number: KE00055

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Overview


Product name:
Human ST2 ELISA Kit

Tests:
1 X 96 well plate

Sample type:
Serum, Plasma, Cell culture supernatant

Assay type:
Sandwich

Sensitivity:
0.12 ng/mL

Range:
0.25-16 ng/mL

Reacted Species:
Human

Tested applications:
Sandwich ELISA

Recovery:
Sample TypeAverageRange
Plasma 101% 77%-127%
Cell culture supernatant 90% 82%-102%

Product overview:
KE00055 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The ST2 ELISA kit is to be used to detect and quantify protein levels of endogenous ST2. The assay recognizes human ST2. A polyclonal antibody specific for ST2 has been pre-coated onto the microwells. The ST2 protein in samples is captured by the coated antibody after incubation. Following extensive washing, a monoclonal antibody specific for ST2 is added to detect the captured ST2 protein. For signal development, horseradish peroxidase (HRP)-conjugated Anti-mouse antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm.

Properties


Storage Instructions:
All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.

Synonyms:
DER4, FIT 1, IL1RL1, interleukin 1 receptor like 1, Protein ST2, ST2, ST2L, ST2V, T1
Background

Protein ST2, also known as IL1RL1, DER4 and T1, is a member of the interleukin 1 receptor family. The gene of ST2 encodes three splice variants: a soluble secreted form, a transmembrane form and a form of undetermined localization. In some ELISA study on patients with autoimmune diseases, the hST2 concentration of some, but not all, sera of patients with systemic lupus erythematosus (SLE), rheumatoid arthritis (RA), Wegener’s granulomatosis, and Behc¸et disease were markedly higher than those of healthy controls. ST2 protein should be important both to fundamental researches and the further clarification of the clinical implications of the ST2 protein.


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