Human TF ELISA Kit0 Publications

Catalog number: KE00046



96 T


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Product name:
Human TF ELISA Kit

1 X 96 well plate

Sample type:
Serum, Plasma, Cell culture supernatant, Urine

Assay type:

0.6 ng/mL

1.56-100 ng/mL

Reacted Species:

Tested applications:
Sandwich ELISA

Sample TypeAverageRange
Cell culture supernatant 102% 90%-120%
Urine 88% 79%-97%
Human plasma 103% 81%-126%

Samplenmean (ng/mL)SDCV%
1 20 111.4 5.6 5.0
2 20 18.4 0.8 4.2
3 20 4.5 0.2 5.3

Samplenmean (ng/mL)SDCV%
1 24 101.3 8.2 8.1
2 24 17.9 1.3 7.2
3 24 4.3 0.3 7.5

Product overview:
KE00046 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The TF ELISA kit is to be used to detect and quantify protein levels of endogenous TF. The assay recognizes human TF. A polyclonal antibody specific for TF has been pre-coated onto the microwells. The TF protein in samples is captured by the coated antibody after incubation. Following extensive washing, a monoclonal antibody specific for TF is added to detect the captured TF protein. For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm.


Storage Instructions:
All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.

Beta 1 metal binding globulin, PRO1557, PRO2086, Serotransferrin, Siderophilin, TF, transferrin

Serotransferrin (TF) is a 77 kDa secreted protein which is also named as transferrin, siderophilin, beta-1 metal-binding globulin and belongs to a family of homologous iron-binding glycoproteins that encompasses lactoferrin (found both intracellular and in secretions, including milk), melanotrasferrin (present on melanoma cells) and ovotransferrin (present in egg white). TF is a multi-function protein with a primary role in transporting iron in a safe, redox-inactive state from absorption to utilization or storage sites around the body. The association of Tf with the immune system derives from its ability to restrict serum free-iron levels, creating low-iron environments where the infection capacity of pathogenic microorganisms is limited. TF is expressed predominantly in the liver and secreted in plasma, but lower amounts can be synthesized in other tissues such as the brain and the testis.

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