Human TLE3 ELISA Kit0 Publications

Catalog number: KE00048


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Product name:
Human TLE3 ELISA Kit

1 X 96 well plate

Sample type:
Plasma,Cell culture supernatant,Urine

Assay type:

11 pg/mL

62.5-4000 pg/mL

Reacted Species:

Tested applications:
Sandwich ELISA

Sample TypeAverageRange
Plasma 97% 82%-126%
Cell culture supernatant 106% 83%-127%
Urine 90% 78%-131%

Samplenmean (pg/mL)SDCV%
1 20 ? ug/ml ? ?%
2 20 ? ug/ml ? ?%
3 20 ? ug/ml ? ?%

Samplenmean (pg/mL)SDCV%
1 20 ? ug/ml ? ?%
2 20 ? ug/ml ? ?%
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Product overview:
KE00048 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The TLE3 ELISA kit is to be used to detect and quantify protein levels of endogenous TLE3. The assay recognizes human TLE3. A polyclonal antibody specific for TLE3 has been pre-coated onto the microwells. The TLE3 protein in samples is captured by the coated antibody after incubation. Following extensive washing, a monoclonal antibody specific for TLE3 is added to detect the captured TLE3 protein. For signal development, horseradish peroxidase (HRP)-conjugated Anti-mouse antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm.


Storage Instructions:
All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.

ESG, ESG3, GRG3, HsT18976, KIAA1547, TLE3

Transducin-like enhancer of split 3 (TLE3), also named as KIAA1547 and ESG3, belongs to the WD repeat Groucho/TLE family. TLE3 is a transcriptional repressor that interacts with a chromatin complex acting downstream of adenomatous polyposis coil (APC) and β-catenin in the Wnt pathway. TLE3 is expressed in most β-cells and a subset of other endocrine cell types in the pancreas. TLE3 is first identified as a candidate biomarker of taxane sensitivity in breast cancer in a large screen of candidate immunohistochemical (IHC) classifiers in a community cohort study. TLE3 is a member of the transducin-like enhancer of split (TLE) family of proteins that have been implicated in the tumorgenesis and classification of sarcomas.

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