Human Uteroglobin ELISA Kit0 Publications

Catalog number: KE00099



96 T


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Product name:
Human Uteroglobin ELISA Kit

1 X 96 well plate

Sample type:
Serum, Plasma, Cell culture supernatant, Urine

Assay type:

0.03 ng/mL

0.156 - 10 ng/mL

Reacted Species:

Tested applications:
Sandwich ELISA

Sample TypeAverageRange
Cell culture supernatant 100% 80%-120%
Urine 99% 83%-119%
Human plasma 100% 78%-121%

Samplenmean (ng/mL)SDCV%
1 20 0.2 0.01 5.4
2 20 2.5 0.18 7.4
3 20 9.8 0.74 7.6

Samplenmean (ng/mL)SDCV%
1 24 0.2 0.02 9.4
2 24 2.6 0.23 7.4
3 24 10.8 1.00 9.2

Product overview:
KE00099 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Uteroglobin ELISA kit is to be used to detect and quantify protein levels of endogenous Uteroglobin. The assay recognizes human Uteroglobin. A polyclonal antibody specific for Uteroglobin has been pre-coated onto the microwells. The Uteroglobin protein in samples is captured by the coated antibody after incubation. Following extensive washing, a monoclonal antibody specific for Uteroglobin is added to detect the captured Uteroglobin protein. For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm.


Storage Instructions:
All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.

CC10, CC16, CCPBP, CCSP, SCGB1A1, UGB, UP 1, UP1, Urinary protein 1, Urine protein 1, Uteroglobin

Uteroglobin, also named clara cell secretory protein or CC16, is mainly secreted by the club cells along the tracheobronchial tree, diffuses passively into the bloodstream and is rapidly eliminated by glomerular filtration. It is a steroid-dependent, immunomodulatory, cytokine-like protein, and it is secreted by the mucosal epithelial cells of many vertebrates and takes part in some signaling pathways. Growing evidence indicates that Uteroglobin has anti-inflammatory and anti-toxicant properties in the lung and may protect against obstructive lung diseases. lower levels of Uteroglobin in blood and airways have been associated with prevalence and severity of Chronic obstructive pulmonary disease (COPD). Defects in Uteroglobin are associated with a susceptibility to asthma.

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