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IGFBP4 Polyclonal antibody

IGFBP4 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat and More (1)

Applications

WB, IHC, IF/ICC, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 18500-1-AP

Print datasheet

Synonyms

IBP4, IBP 4, HT29 IGFBP, Deb2, BP4



经过测试的应用

Positive WB detected inC6 cells
Positive IHC detected inhuman liver tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inC6 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18500-1-AP targets IGFBP4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IHC, IF
Tested Reactivity human, rat
Cited Reactivityhuman, rat, chicken
Immunogen IGFBP4 fusion protein Ag13347 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name insulin-like growth factor binding protein 4
Synonyms IBP4, IBP 4, HT29 IGFBP, Deb2, BP4
Calculated Molecular Weight 28 kDa
Observed Molecular Weight 28-40 kDa
GenBank Accession NumberBC016041
Gene Symbol IGFBP4
Gene ID (NCBI) 3487
RRIDAB_2878547
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP22692
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IGFBP4, also named as IBP4, is a 258 amino acid protein, which contains one IGFBP N-terminal domain and one thyroglobulin type-1 domain. IGFBP4 is the smallest member of the insulin-like growth factor binding protein family (IGFBP). It is a hepatic protein that plays a role in modulating the activity and bioavailability of IGF-I. The expression of IGFBP4 was found to increase under conditions of hypoxia. IGFBP4 is highly expressed in adipocytes and osteoblasts and is inhibitory of IGFs in vitro. (PMID: 28938423, PMID: 34646401). IGFBP4 is a secreted protein and calculated molecular weight is 28 kDa.

实验方案

Product Specific Protocols
WB protocol for IGFBP4 antibody 18500-1-APDownload protocol
IHC protocol for IGFBP4 antibody 18500-1-APDownload protocol
IF protocol for IGFBP4 antibody 18500-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Sci Adv

The CCL2-CCR2 astrocyte-cancer cell axis in tumor extravasation at the brain.

Authors - Cynthia Hajal
humanWB

Theranostics

Proteomic analysis of intracellular protein corona of nanoparticles elucidates nano-trafficking network and nano-bio interactions.

Authors - Mengmeng Qin
humanWB

BMC Biol

SMYD3 associates with the NuRD (MTA1/2) complex to regulate transcription and promote proliferation and invasiveness in hepatocellular carcinoma cells

Authors - Yang Yang
humanIHC

Front Oncol

High Serpin Family A Member 10 Expression Confers Platinum Sensitivity and Is Associated With Survival Benefit in High-Grade Serous Ovarian Cancer: Based on Quantitative Proteomic Analysis.

Authors - Wenwen Guo
chickenWB

Cell Signal

PMID: 28734787

Authors - Jie Yang
humanWB

Oral Dis

FERMT2 upregulation in CAFs enhances EMT of OSCC and M2 macrophage polarization

Authors - Xiangrui Ma