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验证数据展示

  • WB analysis using 27163-1-AP

    WB analysis using 27163-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27163-1-AP (IL23R antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 27163-1-AP

    IHC staining of human tonsillitis using 27163-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 27163-1-AP (IL23R antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human tonsillitis using 27163-1-AP

    IHC staining of human tonsillitis using 27163-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 27163-1-AP (IL23R antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

IL-23R Polyclonal antibody

IL-23R Polyclonal Antibody for WB, IHC, ELISA
Cat No. 27163-1-AP
Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, ELISA

Synonyms

IL23R, Interleukin-23 receptor, interleukin 23 receptor, IL-23 receptor, IL 23R

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inJurkat cells, A431 cells, RAW 264.7 cells, Raji cells, THP-1 cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

27163-1-AP targets IL-23R in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse
Cited Reactivitymouse, rat
Immunogen IL-23R fusion protein Ag25939 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name interleukin 23 receptor
Synonyms IL23R, Interleukin-23 receptor, interleukin 23 receptor, IL-23 receptor, IL 23R
Calculated Molecular Weight 72 kDa
Observed Molecular Weight 65-75 kDa
GenBank Accession Number NM_144701
Gene Symbol IL-23R
Gene ID (NCBI) 149233
RRIDAB_2880783
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Interleukin-23 receptor (IL-23R) is a type I cytokine receptor that plays a critical role in immune responses and inflammation. It forms a receptor complex with IL-12Rβ1 and is activated by the cytokine IL-23, which is primarily produced by dendritic cells and activated macrophages (PMID: 39796684). The genetic and epigenetic interactions in the IL-23R/IL-17 axis are associated with elevated expression of IL-17 and inflammatory bowel disease (IBD) pathogenesis (PMID: 21672939).

实验方案

Product Specific Protocols
WB protocol for IL-23R antibody 27163-1-APDownload protocol
IHC protocol for IL-23R antibody 27163-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Front Immunol

IL-17 Aggravates Pseudomonas aeruginosa Airway Infection in Acute Exacerbations of Chronic Obstructive Pulmonary Disease.

Authors - Fengming Ding
View Article
ratWB

Naunyn Schmiedebergs Arch Pharmacol

Short-term pretreatment of naringin isolated from Citrus wilsonii Tanaka attenuates rat myocardial ischemia/reperfusion injury.

Authors - Wenting Liu
View Article