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验证数据展示

  • WB analysis of HeLa using 12750-1-AP

    WB analysis of HeLa using 12750-1-AP

    WB result of IMP3 antibody (12750-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-IMP3 transfected HeLa cells.

  • WB analysis using 12750-1-AP

    WB analysis using 12750-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12750-1-AP (IMP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 12750-1-AP

    IP experiment of HeLa using 12750-1-AP

    IP result of anti-IMP3 (IP:12750-1-AP, 3ug; Detection:12750-1-AP 1:500) with HeLa cells lysate 1200ug.

  • IHC staining of human ovary cancer using 12750-1-AP

    IHC staining of human ovary cancer using 12750-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 12750-1-AP (IMP3 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 12750-1-AP

    IF Staining of HepG2 using 12750-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using IMP3 antibody (12750-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Featured Product
  • KD/KO验证

IMP3 Polyclonal antibody

IMP3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 12750-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, ELISA

别名

MRPS4, IMP 3, C15orf12, BRMS2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, MCF-7 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12750-1-AP targets IMP3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原 IMP3 fusion protein Ag3477 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 IMP3, U3 small nucleolar ribonucleoprotein, homolog (yeast)
别名 MRPS4, IMP 3, C15orf12, BRMS2
计算分子量 184 aa, 22 kDa
观测分子量 22 kDa
GenBank蛋白编号BC020557
基因名称 IMP3
Gene ID (NCBI) 55272
RRIDAB_2122784
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NV31
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IMP U3 small nucleolar ribonucleoprotein 3 (IMP3, BRMS2), is a component of U3 small nucleolar ribonucleoprotein complex (U3 snoRNP), has been identified as a participant in pre-rRNA processing for nearly twenty years.

实验方案

Product Specific Protocols
WB protocol for IMP3 antibody 12750-1-APDownload protocol
IHC protocol for IMP3 antibody 12750-1-APDownload protocol
IF protocol for IMP3 antibody 12750-1-APDownload protocol
IP protocol for IMP3 antibody 12750-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,mouseWB,IHC

Oncotarget

IMP2/IGF2BP2 expression, but not IMP1 and IMP3, predicts poor outcome in patients and high tumor growth rate in xenograft models of gallbladder cancer.

Authors - Sonja M Kessler
View Article