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验证数据展示

  • WB analysis of A549 using 66835-1-Ig

    WB analysis of A549 using 66835-1-Ig

    A549 cells were subjected to SDS PAGE followed by western blot with 66835-1-Ig (IRF5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

IRF5 Monoclonal antibody

IRF5 Monoclonal Antibody for WB, ELISA
Cat No. 66835-1-Ig
CloneNo. 3A4A10

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse

Applications

WB, ELISA

Synonyms

interferon regulatory factor 5, IRF 5, IRF5, SLEB10

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, HeLa cells, THP-1 cells, RAW 264.7 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66835-1-Ig targets IRF5 in WB, ELISA applications and shows reactivity with Human, mouse samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human, mouse
Immunogen IRF5 fusion protein Ag5442 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name interferon regulatory factor 5
Synonyms interferon regulatory factor 5, IRF 5, IRF5, SLEB10
Calculated Molecular Weight 56 kDa
Observed Molecular Weight 56 kDa
GenBank Accession NumberBC004201
Gene Symbol IRF5
Gene ID (NCBI) 3663
RRIDAB_2882178
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IRF5, also named as SLEB10, contians one IRF tryptophan pentad repeat DNA-binding domain and belongs to the IRF family. It is a transcription factor involved in the induction of interferons IFNA and INFB and inflammatory cytokines upon virus infection. It is activated by TLR7 or TLR8 signaling. Genetic variations in IRF5 are associated with susceptibility to inflammatory bowel disease type 14 (IBD14) and systemic lupus erythematosus type 10 (SLEB10). IRF5 is also used as M1 marcrophage lineage marker. Alternative splice variant encoding different isoforms exist.

实验方案

Product Specific Protocols
WB protocol for IRF5 antibody 66835-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols