验证数据展示
经过测试的应用
Positive WB detected in | A549 cells, HeLa cells, THP-1 cells, RAW 264.7 cells |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:1000-1:6000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
66835-1-Ig targets IRF5 in WB, ELISA applications and shows reactivity with Human, mouse samples.
Tested Applications | WB, ELISA Application Description |
Tested Reactivity | Human, mouse |
Immunogen | IRF5 fusion protein Ag5442 种属同源性预测 |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Full Name | interferon regulatory factor 5 |
Synonyms | interferon regulatory factor 5, IRF 5, IRF5, SLEB10 |
Calculated Molecular Weight | 56 kDa |
Observed Molecular Weight | 56 kDa |
GenBank Accession Number | BC004201 |
Gene Symbol | IRF5 |
Gene ID (NCBI) | 3663 |
RRID | AB_2882178 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
IRF5, also named as SLEB10, contians one IRF tryptophan pentad repeat DNA-binding domain and belongs to the IRF family. It is a transcription factor involved in the induction of interferons IFNA and INFB and inflammatory cytokines upon virus infection. It is activated by TLR7 or TLR8 signaling. Genetic variations in IRF5 are associated with susceptibility to inflammatory bowel disease type 14 (IBD14) and systemic lupus erythematosus type 10 (SLEB10). IRF5 is also used as M1 marcrophage lineage marker. Alternative splice variant encoding different isoforms exist.
实验方案
Product Specific Protocols | |
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WB protocol for IRF5 antibody 66835-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |