验证数据展示
经过测试的应用
| Positive WB detected in | HT-1080 cells, HUVEC cells |
Planning an IHC experiment? We recommend our IHCeasy ITGB1/CD29 Ready-To-Use IHC Kit. ITGB1/CD29 primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
66315-1-Ig targets Integrin Beta 1 in WB, ELISA applications and shows reactivity with human samples.
| Tested Applications | WB, ELISA Application Description |
| Cited Applications | WB |
| Tested Reactivity | human |
| Cited Reactivity | human |
| Immunogen | Integrin Beta 1 fusion protein Ag24476 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12) |
| Synonyms | CD29, FNRB, GPIIA, Integrin beta 1, Integrin beta-1, ITGB1, MDF2, MSK12, VLA 4 subunit beta, VLA BETA, VLAB |
| Calculated Molecular Weight | 88 kDa |
| Observed Molecular Weight | 130 kDa |
| GenBank Accession Number | BC020057 |
| Gene Symbol | Integrin beta 1 |
| Gene ID (NCBI) | 3688 |
| ENSEMBL Gene ID | ENSG00000150093 |
| RRID | AB_2881696 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P05556 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Integrin beta-1 (ITGB1), also named as CD29, is a 130 kDa single chain type I glycoprotein that is expressed in a heterodimeric complex with one of six distinct α subunits, comprising the very late activation antigen (VLA) subfamily of adhesion receptors. It is one of the essential surface molecules expressed on human MSC from bone marrow and other sources. The β1 subunit is also broadly expressed on lymphocytes and monocytes, weakly expressed on granulocytes, and not expressed on erythrocytes. These receptors are involved in a variety of cell-cell and cell-matrix interactions.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Integrin Beta 1 antibody 66315-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
