验证数据展示

IF Staining of Jurkat using 24164-1-AP
Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using JNK antibody (24164-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
WB analysis using 24164-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 24164-1-AP (JNK antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis using 24164-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 24164-1-AP (JNK antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
IHC staining of human colon cancer using 24164-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 24164-1-AP (JNK antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with MAPK8/JNK antibody included
IHC staining of human colon cancer using 24164-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 24164-1-AP (JNK antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with MAPK8/JNK antibody included
FC experiment of HeLa using 24164-1-AP
1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human JNK (24164-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
IP experiment of A431 using 24164-1-AP
IP result of anti-JNK (IP:24164-1-AP, 4ug; Detection:24164-1-AP 1:10000) with A431 cells lysate 1280 ug.

JNK Polyclonal antibody

JNK Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Cat No. 24164-1-AP

产品说明书

引用文献(175)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat and More (1)

应用

WB, IHC, IF/ICC, FC (Intra), IP, ELISA and More (2)

别名

MAPK8, JNK1A2, JNK1, JNK 2, JNK 1

缓冲液配方: PBS and Azide

PBS and Azide

偶联物: Unconjugated

Unconjugated

CoraLite® Plus 488

规格:

-/ -

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概述

经过测试的应用
产品信息
发表文章 (175)
实验方案

产品说明书

MSDS

经过测试的应用

Positive WB detected in	A431 cells, Jurkat cells, NIH/3T3 cells, HEK-293 cells, HeLa cells, Neuro-2a cells, mouse brain tissue, rat brain tissue
Positive IP detected in	A431 cells
Positive IHC detected in	human colon cancer tissue, human stomach cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	Jurkat cells
Positive FC (Intra) detected in	HeLa cells

Planning an IHC experiment? We recommend our IHCeasy MAPK8/JNK Ready-To-Use IHC Kit. MAPK8/JNK primary antibody included.

Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

应用	推荐稀释比
Western Blot (WB)	WB : 1:2000-1:16000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WB	See 171 publications below
IHC	See 5 publications below
IF	See 2 publications below
CoIP	See 1 publications below

产品信息

24164-1-AP targets JNK in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用	WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
文献引用应用	WB, IHC, IF, CoIP
经测试反应性	human, mouse, rat
文献引用反应性	human, mouse, rat, pig
免疫原	JNK fusion protein Ag21426 种属同源性预测
宿主/亚型	Rabbit / IgG
抗体类别	Polyclonal
产品类型	Antibody
全称	mitogen-activated protein kinase 8
别名	MAPK8, JNK1A2, JNK1, JNK 2, JNK 1
计算分子量	48 kDa
观测分子量	42 kDa, 50 kDa
GenBank蛋白编号	BC130572
基因名称	JNK
Gene ID (NCBI)	5599
RRID	AB_2879443
偶联类型	Unconjugated
形式	Liquid
纯化方式	Antigen affinity purification
UNIPROT ID	P45983
储存缓冲液	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

Function

JNK1, also known as mitogen-activated protein kinase 8 - MAPK8, is a serine/threonine-protein kinase. It is an important player in signaling transduction pathways governing cellular response to external stimuli, including ultraviolet radiation (UV), inflammatory cytokines, and reactive oxygen species (ROS). JNK1 phosphorylates several transcription factors, such as c-Jun, Elk-1, and ATF2, affecting their transcription activity.

Tissue specificity

JNK1 is ubiquitously expressed.

Involvement in disease

JNK-mediated phosphorylation governs a variety of important cellular processes, and abnormalities of JNK1 activity have been implicated in obesity and cancer.

Isoforms

There are four isoforms of JNK1 (PMID: 8654373), giving rise to 46 kDa and 55 kDa isoforms. JNK1 isoforms differ in their substrate specificity and tissue expression profiles.

Post-translational modifications

JNK1 is phosphorylated by MAPK kinases (MAPKK).

Cellular localization

JNK1 is present both in the nucleus and in the cytoplasm. Stress conditions and other stimuli can cause a shift in JNK1 subcellular distribution.

实验方案

Product Specific Protocols
WB protocol for JNK antibody 24164-1-AP	Download protocol
IHC protocol for JNK antibody 24164-1-AP	Download protocol
IF protocol for JNK antibody 24164-1-AP	Download protocol
IP protocol for JNK antibody 24164-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
	WB	Mol Cell PARP5A and RNF146 phase separation restrains RIPK1-dependent necroptosis Authors - Shouqiao Hou View Article
human	WB	Adv Sci (Weinh) OR11H1 Missense Variant Confers the Susceptibility to Vogt-Koyanagi-Harada Disease by Mediating Gadd45g Expression Authors - Xingran Li View Article
mouse	WB	Redox Biol FUT2-dependent fucosylation of HYOU1 protects intestinal stem cells against inflammatory injury by regulating unfolded protein response Authors - Zhe Wang View Article
mouse	WB	Clin Transl Med PINK1 modulates Prdx2 to reduce lipotoxicity-induced apoptosis and attenuate cardiac dysfunction in heart failure mice with a preserved ejection fraction Authors - Hao Zhang View Article
mouse	WB	Transl Res Mitochondria transplantation protects traumatic brain injury via promoting neuronal survival and astrocytic BDNF. Authors - Jiqian Zhao View Article
human	WB	J Immunother Cancer TSH-TSHR axis promotes tumor immune evasion. Authors - Zhenghao Wu View Article

验证数据展示

IF Staining of Jurkat using 24164-1-AP

WB analysis using 24164-1-AP

WB analysis using 24164-1-AP

IHC staining of human colon cancer using 24164-1-AP

IHC staining of human colon cancer using 24164-1-AP

FC experiment of HeLa using 24164-1-AP

IP experiment of A431 using 24164-1-AP

JNK Polyclonal antibody

JNK Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

-/ -

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

Function

Tissue specificity

Involvement in disease

Isoforms

Post-translational modifications

Cellular localization

实验方案

发表文章

PARP5A and RNF146 phase separation restrains RIPK1-dependent necroptosis

OR11H1 Missense Variant Confers the Susceptibility to Vogt-Koyanagi-Harada Disease by Mediating Gadd45g Expression

FUT2-dependent fucosylation of HYOU1 protects intestinal stem cells against inflammatory injury by regulating unfolded protein response

PINK1 modulates Prdx2 to reduce lipotoxicity-induced apoptosis and attenuate cardiac dysfunction in heart failure mice with a preserved ejection fraction

Mitochondria transplantation protects traumatic brain injury via promoting neuronal survival and astrocytic BDNF.

TSH-TSHR axis promotes tumor immune evasion.