验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HCT 116 cells, HepG2 cells, A549 cells, HEK-293 cells, Caco-2 cells, MOLT-4 cells, TF-1 cells, RT-4 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human breast cancer tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:20000-1:100000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
66630-1-Ig targets KAP1 in WB, IHC, IF/ICC, IP, ChIP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IF, ChIP |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse |
| 免疫原 | KAP1 fusion protein Ag7519 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2a |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | tripartite motif-containing 28 |
| 别名 | TRIM28, KRAB associated protein 1, KAP-1, KAP 1, EC:2.3.2.27 |
| 计算分子量 | 89 kDa |
| 观测分子量 | 100 kDa |
| GenBank蛋白编号 | BC004978 |
| 基因名称 | KAP1 |
| Gene ID (NCBI) | 10155 |
| RRID | AB_2732886 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q13263 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
KAP1, also named as TRIM28 or RNF96, is a 835 amino acid protein, which contain one RING-type zinc finger, one PHD-type zinc finger, one bromo domain and two B box-type zinc fingers. KAP1 localizes in the nucleus and Belongs to the TRIM/RBCC family. KAP1 is a nuclear corepressor for KRAB domain-containing zinc finger proteins and mediates gene silencing by recruiting CHD3, a subunit of the nucleosome remodeling and deacetylation (NuRD) complex, and SETDB1 to the promoter regions of KRAB target genes. KAP1 is expressed in all tissues tested including spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocytes. The calcualted molecular weight of KAP1 is 89 kDa, but modified KAP1 is about 100 kDa. (PMID: 18590578 )
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for KAP1 antibody 66630-1-Ig | Download protocol |
| IHC protocol for KAP1 antibody 66630-1-Ig | Download protocol |
| IF protocol for KAP1 antibody 66630-1-Ig | Download protocol |
| IP protocol for KAP1 antibody 66630-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Sci Adv Epac1 activation by cAMP regulates cellular SUMOylation and promotes the formation of biomolecular condensates. | ||
Nucleic Acids Res SIRT6 coordinates with CHD4 to promote chromatin relaxation and DNA repair. | ||
BMC Pulm Med miR-125b-5p upregulation by TRIM28 induces cisplatin resistance in non-small cell lung cancer through CREB1 inhibition
| ||
Open Biol A critical threshold of MCM10 is required to maintain genome stability during differentiation of induced pluripotent stem cells into natural killer cells | ||
