PCAF Polyclonal antibody

PCAF Polyclonal Antibody for IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IP, IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 13983-1-AP

Print datasheet

Synonyms

CAF, Histone acetylase PCAF, Histone acetyltransferase PCAF, K(lysine) acetyltransferase 2B, KAT2B, Lysine acetyltransferase 2B, P/CAF, P300/CBP associated factor, PCAF



经过测试的应用

Positive IP detected inHeLa cells
Positive IHC detected inhuman heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 4 publications below

产品信息

13983-1-AP targets PCAF in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IP, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen PCAF fusion protein Ag5028 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name K(lysine) acetyltransferase 2B
Synonyms CAF, Histone acetylase PCAF, Histone acetyltransferase PCAF, K(lysine) acetyltransferase 2B, KAT2B, Lysine acetyltransferase 2B, P/CAF, P300/CBP associated factor, PCAF
Calculated Molecular Weight 93 kDa
GenBank Accession NumberBC060823
Gene Symbol PCAF/KAT2B
Gene ID (NCBI) 8850
RRIDAB_10643392
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ92831
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for PCAF antibody 13983-1-APDownload protocol
IP protocol for PCAF antibody 13983-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nat Commun

Phosphoglycerate dehydrogenase activates PKM2 to phosphorylate histone H3T11 and attenuate cellular senescence

Authors - Yinsheng Wu
humanWB

Mol Cell Proteomics

Acetylproteomic analysis reveals functional implications of lysine acetylation in human spermatozoa (sperm).

Authors - Heguo Yu
humanWB

Exp Cell Res

GRIM-19 inhibits proliferation and induces apoptosis in a p53-dependent manner in colorectal cancer cells through the SIRT7/PCAF/MDM2 axis.

Authors - Ding Wang
humanWB

PLoS One

Psychostimulants and opioids differentially influence the epigenetic modification of histone acetyltransferase and histone deacetylase in astrocytes.

Authors - Mayur Doke