验证数据展示

WB analysis of HeLa using 12345-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 12345-1-AP (DYNC1H1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis using 12345-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 12345-1-AP (DYNC1H1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
IF Staining of MCF-7 using 12345-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using DYNC1H1 antibody (12345-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).
IHC staining of mouse brain using 12345-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12345-1-AP (DYNC1H1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with DYNC1H1 antibody included
IHC staining of mouse brain using 12345-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12345-1-AP (DYNC1H1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with DYNC1H1 antibody included
IP experiment of HeLa using 12345-1-AP
IP result of anti-DYNC1H1 (IP:12345-1-AP, 4ug; Detection:12345-1-AP 1:5000) with HeLa cells lysate 1085 ug.
IHC staining of human normal colon using 12345-1-AP
Immunohistochemical analysis of paraffin-embedded human normal colon slide using 12345-1-AP (DYNC1H1 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with DYNC1H1 antibody included
IHC staining of human colon using 12345-1-AP
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 12345-1-AP (DYNC1H1 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with DYNC1H1 antibody included

Featured Product
KD/KO Validated

DYNC1H1 Polyclonal antibody

DYNC1H1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 12345-1-AP

Publications(55)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, zebrafish and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA and More (2)

Synonyms

DYNC1H 1

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488

Size:

-/ -

点击咨询售前客服

点击咨询售后客服

概述

经过测试的应用
产品信息
发表文章 (55)
实验方案

Print datasheet

MSDS

经过测试的应用

Positive WB detected in	HeLa cells, human brain tissue, mouse brain tissue, Jurkat cells
Positive IP detected in	HeLa cells
Positive IHC detected in	mouse brain tissue, human breast cancer tissue, human normal colon, human testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	MCF-7 cells

Planning an IHC experiment? We recommend our IHCeasy DYNC1H1 Ready-To-Use IHC Kit. DYNC1H1 primary antibody included.

Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:12000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

KD/KO	See 6 publications below
WB	See 33 publications below
IHC	See 6 publications below
IF	See 16 publications below
IP	See 3 publications below
CoIP	See 1 publications below

产品信息

12345-1-AP targets DYNC1H1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat, zebrafish samples.

Tested Applications	WB, IHC, IF/ICC, IP, ELISA Application Description
Cited Applications	WB, IHC, IF, IP, CoIP
Tested Reactivity	human, mouse, rat, zebrafish
Cited Reactivity	human, mouse, rat, pig, zebrafish
Immunogen	DYNC1H1 fusion protein Ag2999 种属同源性预测
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Full Name	dynein, cytoplasmic 1, heavy chain 1
Synonyms	DYNC1H 1
Calculated Molecular Weight	4646 aa, 532 kDa
Observed Molecular Weight	532 kDa
GenBank Accession Number	BC021297
Gene Symbol	DYNC1H1
Gene ID (NCBI)	1778
RRID	AB_2261765
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q14204
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

Dyneins are a group of microtubule-activated ATPases that serve to convert chemical energy into mechanical energy. It can be divided into 2 large subgroups, namely, the axonemal and cytoplasmic dyneins. The conventional cytoplasmic dynein are comprised of 2 heavy chain polypeptides and a number of intermediate and light chains. DYNC1H1 is a cytoplasmic dynein and belongs to the dynein heavy chain family. It acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. DYNC1H1 has been implicated in the degeneration of dopaminergic neuron axons and motor neurons in PD patients..

实验方案

Product Specific Protocols
WB protocol for DYNC1H1 antibody 12345-1-AP	Download protocol
IHC protocol for DYNC1H1 antibody 12345-1-AP	Download protocol
IF protocol for DYNC1H1 antibody 12345-1-AP	Download protocol
IP protocol for DYNC1H1 antibody 12345-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
mouse	IF	Science Mechanism of spindle pole organization and instability in human oocytes. Authors - Chun So View Article
mouse	IF	Science A liquid-like spindle domain promotes acentrosomal spindle assembly in mammalian oocytes. Authors - Chun So View Article
human	WB	Nature Endophilin-A2 functions in membrane scission in clathrin-independent endocytosis. Authors - Henri-François Renard KD Validated View Article
human	WB	Nat Cell Biol Recycling of autophagosomal components from autolysosomes by the recycler complex. Authors - Chuchu Zhou View Article
human		Neuron Posterior Neocortex-Specific Regulation of Neuronal Migration by CEP85L Identifies Maternal Centriole-Dependent Activation of CDK5. Authors - Andrew Kodani View Article
human	WB	J Cell Biol Ndel1 suppresses ciliogenesis in proliferating cells by regulating the trichoplein-Aurora A pathway. Authors - Hironori Inaba KD Validated View Article

验证数据展示

WB analysis of HeLa using 12345-1-AP

WB analysis using 12345-1-AP

IF Staining of MCF-7 using 12345-1-AP

IHC staining of mouse brain using 12345-1-AP

IHC staining of mouse brain using 12345-1-AP

IP experiment of HeLa using 12345-1-AP

IHC staining of human normal colon using 12345-1-AP

IHC staining of human colon using 12345-1-AP

DYNC1H1 Polyclonal antibody

DYNC1H1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

Mechanism of spindle pole organization and instability in human oocytes.

A liquid-like spindle domain promotes acentrosomal spindle assembly in mammalian oocytes.

Endophilin-A2 functions in membrane scission in clathrin-independent endocytosis.

Recycling of autophagosomal components from autolysosomes by the recycler complex.

Posterior Neocortex-Specific Regulation of Neuronal Migration by CEP85L Identifies Maternal Centriole-Dependent Activation of CDK5.

Ndel1 suppresses ciliogenesis in proliferating cells by regulating the trichoplein-Aurora A pathway.