验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, human brain tissue, mouse brain tissue, Jurkat cells |
Positive IP detected in | HeLa cells |
Positive IHC detected in | mouse brain tissue, human breast cancer tissue, human normal colon, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | MCF-7 cells |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:2000-1:12000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
12345-1-AP targets DYNC1H1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat, zebrafish samples.
Tested Applications | WB, IHC, IF/ICC, IP, ELISA Application Description |
Cited Applications | WB, IHC, IF, IP, CoIP |
Tested Reactivity | human, mouse, rat, zebrafish |
Cited Reactivity | human, mouse, rat, pig, zebrafish |
Immunogen | DYNC1H1 fusion protein Ag2999 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | dynein, cytoplasmic 1, heavy chain 1 |
Synonyms | DYNC1H 1 |
Calculated Molecular Weight | 4646 aa, 532 kDa |
Observed Molecular Weight | 532 kDa |
GenBank Accession Number | BC021297 |
Gene Symbol | DYNC1H1 |
Gene ID (NCBI) | 1778 |
RRID | AB_2261765 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q14204 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Dyneins are a group of microtubule-activated ATPases that serve to convert chemical energy into mechanical energy. It can be divided into 2 large subgroups, namely, the axonemal and cytoplasmic dyneins. The conventional cytoplasmic dynein are comprised of 2 heavy chain polypeptides and a number of intermediate and light chains. DYNC1H1 is a cytoplasmic dynein and belongs to the dynein heavy chain family. It acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. DYNC1H1 has been implicated in the degeneration of dopaminergic neuron axons and motor neurons in PD patients..
实验方案
Product Specific Protocols | |
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WB protocol for DYNC1H1 antibody 12345-1-AP | Download protocol |
IHC protocol for DYNC1H1 antibody 12345-1-AP | Download protocol |
IF protocol for DYNC1H1 antibody 12345-1-AP | Download protocol |
IP protocol for DYNC1H1 antibody 12345-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
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Science A liquid-like spindle domain promotes acentrosomal spindle assembly in mammalian oocytes. | ||
Nature Endophilin-A2 functions in membrane scission in clathrin-independent endocytosis.
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Nat Cell Biol Recycling of autophagosomal components from autolysosomes by the recycler complex. | ||
Neuron Posterior Neocortex-Specific Regulation of Neuronal Migration by CEP85L Identifies Maternal Centriole-Dependent Activation of CDK5. | ||
J Cell Biol Ndel1 suppresses ciliogenesis in proliferating cells by regulating the trichoplein-Aurora A pathway.
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