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- KD/KO Validated
KRAS Polyclonal antibody
KRAS Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (2)
Applications
WB, IHC, IF/ICC, FC (Intra), IP, ELISA and More (1)
Conjugate
Unconjugated
133
验证数据展示
经过测试的应用
Positive WB detected in | HEK-293 cells, human brain tissue, human kidney tissue, mouse kidney tissue, rat kidney tissue, A549 cells, rat liver tissue, HeLa cells, mouse brain tissue, rat brain tissue |
Positive IP detected in | HeLa cells |
Positive IHC detected in | human lung cancer tissue, human kidney tissue, human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HeLa cells |
Positive FC (Intra) detected in | HeLa cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
12063-1-AP targets KRAS in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description |
Cited Applications | WB, IHC, IF, IP |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat, chicken, zebrafish |
Immunogen | KRAS fusion protein Ag2700 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog |
Synonyms | KRAS4A, KRAS4B, pan RAS, GTPase KRas, EC:3.6.5.2 |
Calculated Molecular Weight | 188 aa, 21 kDa |
Observed Molecular Weight | 21 kDa |
GenBank Accession Number | BC013572 |
Gene Symbol | KRAS |
Gene ID (NCBI) | 3845 |
RRID | AB_878040 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P01116 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for KRAS antibody 12063-1-AP | Download protocol |
IHC protocol for KRAS antibody 12063-1-AP | Download protocol |
IF protocol for KRAS antibody 12063-1-AP | Download protocol |
IP protocol for KRAS antibody 12063-1-AP | Download protocol |
FC protocol for KRAS antibody 12063-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Nat Biotechnol Conformation-locking antibodies for the discovery and characterization of KRAS inhibitors. | ||
Cancer Discov Systematic functional interrogation of rare cancer variants identifies oncogenic alleles. | ||
Mol Cell The oncomicropeptide APPLE promotes hematopoietic malignancy by enhancing translation initiation. | ||