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KRAS Polyclonal antibody

KRAS Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 12063-1-AP

Print datasheet

Synonyms

KRAS4A, KRAS4B, pan RAS, GTPase KRas, EC:3.6.5.2



经过测试的应用

Positive WB detected inHEK-293 cells, human brain tissue, human kidney tissue, mouse kidney tissue, rat kidney tissue, A549 cells, rat liver tissue, HeLa cells, mouse brain tissue, rat brain tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman lung cancer tissue, human kidney tissue, human pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12063-1-AP targets KRAS in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
Cited ApplicationsWB, IHC, IF, IP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, chicken, zebrafish
Immunogen KRAS fusion protein Ag2700 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog
Synonyms KRAS4A, KRAS4B, pan RAS, GTPase KRas, EC:3.6.5.2
Calculated Molecular Weight 188 aa, 21 kDa
Observed Molecular Weight 21 kDa
GenBank Accession NumberBC013572
Gene Symbol KRAS
Gene ID (NCBI) 3845
RRIDAB_878040
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP01116
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

KRAS, also called p21, is a member of the Ras superfamily of proteins. It is located on human chromosome 12, and contains four coding exons and a 5' non-coding exon (PMID: 12778136). KRAS is a membrane-anchored guanosine triphosphate/guanosine diphosphate (GTP/GDP)-binding protein and is widely expressed in most human cells. Like other members of the Ras family, the KRAS protein is a GTPase, and it is involved in intracellular signal transduction and mainly responsible for EGFR-signaling activation (PMID: 19117687). KRAS mutations have been found in various malignancies, including lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma.

实验方案

Product Specific Protocols
WB protocol for KRAS antibody 12063-1-APDownload protocol
IHC protocol for KRAS antibody 12063-1-APDownload protocol
IF protocol for KRAS antibody 12063-1-APDownload protocol
IP protocol for KRAS antibody 12063-1-APDownload protocol
FC protocol for KRAS antibody 12063-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nat Biotechnol

Conformation-locking antibodies for the discovery and characterization of KRAS inhibitors.

Authors - Christopher W Davies
humanWB

Cancer Discov

LZTR1 mutation mediates oncogenesis through stabilization of EGFR and AXL

Authors - Aram Ko
mouseWB,IHC

Cell Res

In vivo self-assembled small RNAs as a new generation of RNAi therapeutics.

Authors - Zheng Fu
mouseWB

Cancer Discov

Systematic functional interrogation of rare cancer variants identifies oncogenic alleles.

Authors - Eejung Kim
humanWB

Mol Cell

The oncomicropeptide APPLE promotes hematopoietic malignancy by enhancing translation initiation.

Authors - Linyu Sun
humanWB

Mol Cell

Regulation of GTPase function by autophosphorylation.

Authors - Christian W Johnson