验证数据展示
经过测试的应用
| Positive WB detected in | A431 cells, HeLa cells, K-562 cells, MCF-7 cells |
| Positive IP detected in | A431 cells |
| Positive IHC detected in | human breast cancer tissue, human appendicitis tissue, human colon cancer tissue, human endometrial cancer tissue, human kidney tissue, human liver cancer tissue, human lung cancer tissue, human ovary tumor tissue, human pancreas cancer tissue, human prostate hyperplasia tissue, human renal cell carcinoma tissue, human stomach cancer tissue, mouse liver tissue, rat liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells, HeLa cells |
| Positive FC (Intra) detected in | HeLa cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:20000-1:100000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10830-1-AP targets Cytokeratin 18 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description |
| Cited Applications | WB, IHC, IF |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, pig, monkey, bovine, sheep, goat, duck |
| Immunogen | Cytokeratin 18 fusion protein Ag1260 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | keratin 18 |
| Synonyms | KRT18, keratin 18, Cytokeratin,Keratin, CK-18, CK18 |
| Calculated Molecular Weight | 48 kDa |
| Observed Molecular Weight | 48 kDa |
| GenBank Accession Number | BC000180 |
| Gene Symbol | Cytokeratin 18 |
| Gene ID (NCBI) | 3875 |
| RRID | AB_2133164 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P05783 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. KRT18, also named as CYK18, PIG46 and K18, is the most commonly found members of the intermediate filament gene family. KRT18 is a type I keratin which is found primarily in non squamous epithelia and is present in a majority of adenocarcinomas and ductal carcinomas but not in squamous cell carcinomas.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Cytokeratin 18 antibody 10830-1-AP | Download protocol |
| IHC protocol for Cytokeratin 18 antibody 10830-1-AP | Download protocol |
| IF protocol for Cytokeratin 18 antibody 10830-1-AP | Download protocol |
| IP protocol for Cytokeratin 18 antibody 10830-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
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Cell Stem Cell Injury Induces Endogenous Reprogramming and Dedifferentiation of Neuronal Progenitors to Multipotency. | ||
Hepatology Fat-associated Lymphoid Clusters as Expandable Niches for Ectopic Liver Development. | ||
J Control Release Mesenchymal stem cell exosomes as nanotherapeutics for dry age-related macular degeneration | ||
Acta Biomater A KPV-binding double-network hydrogel restores gut mucosal barrier in an inflamed colon. | ||
Proc Natl Acad Sci U S A Role of estrogen receptor beta in uterine stroma and epithelium: Insights from estrogen receptor beta-/- mice. | ||
Proc Natl Acad Sci U S A Basal epithelial stem cells are efficient targets for prostate cancer initiation. |
