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验证数据展示

  • WB analysis using 17513-1-AP

    WB analysis using 17513-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17513-1-AP (Cytokeratin 7-specific antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 17513-1-AP

    IHC staining of human lung cancer using 17513-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 17513-1-AP (Cytokeratin 7-specific antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Cytokeratin 7 antibody included
  • IHC staining of human lung cancer using 17513-1-AP

    IHC staining of human lung cancer using 17513-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 17513-1-AP (Cytokeratin 7-specific antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Cytokeratin 7 antibody included
  • IP experiment of HepG2 using 17513-1-AP

    IP experiment of HepG2 using 17513-1-AP

    IP result of anti-Cytokeratin 7-specific (IP:17513-1-AP, 4ug; Detection:17513-1-AP 1:1000) with HepG2 cells lysate 3600ug.

  • FC experiment of HeLa using 17513-1-AP

    FC experiment of HeLa using 17513-1-AP

    1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human Cytokeratin 7-specific (17513-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of mouse liver using 17513-1-AP

    IF Staining of mouse liver using 17513-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using Cytokeratin 7-specific antibody (17513-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 17513-1-AP

    IF Staining of HeLa using 17513-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Cytokeratin 7-specific antibody (17513-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Featured Product
  • KD/KO验证

Cytokeratin 7-specific Polyclonal antibody

Cytokeratin 7-specific Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA
Cat No. 17513-1-AP

产品说明书

引用文献(52)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat and More (1)

应用

WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA and More (1)

别名

Cytokeratin 7, KRT7, Cytokeratin-7, Cytokeratin,Keratin, CK-7

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA431 cells, HeLa cells, T-47D cells, mouse bladder tissue, mouse lung tissue, rat bladder tissue, rat lung tissue
Positive IP detected inHepG2 cells
Positive IHC detected inhuman lung cancer tissue, human bowen disease, human breast cancer tissue, human cervical cancer tissue, human kidney tissue, human ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse liver tissue
Positive IF/ICC detected inHeLa cells, HepG2 cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy Cytokeratin 7 Ready-To-Use IHC Kit. Cytokeratin 7 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:10000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17513-1-AP targets Cytokeratin 7-specific in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat, bovine
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 keratin 7
别名 Cytokeratin 7, KRT7, Cytokeratin-7, Cytokeratin,Keratin, CK-7
计算分子量 51 kDa
观测分子量 51 kDa
GenBank蛋白编号NM_005556
基因名称 Cytokeratin 7
Gene ID (NCBI) 3855
RRIDAB_2134468
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP08729
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. KRT7 is a type II keratin. It is specifically expressed in the simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vessels. This antibody is specifically against KRT7.

实验方案

Product Specific Protocols
WB protocol for Cytokeratin 7-specific antibody 17513-1-APDownload protocol
IHC protocol for Cytokeratin 7-specific antibody 17513-1-APDownload protocol
IF protocol for Cytokeratin 7-specific antibody 17513-1-APDownload protocol
IP protocol for Cytokeratin 7-specific antibody 17513-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratIF

Transl Res

SERPINA5 may promote the development of preeclampsia by disruption of the uPA/uPAR pathway.

Authors - Yan Long
View Article
humanIHC

Clin Cancer Res

Integrative Genomic Analysis of Gemcitabine Resistance in Pancreatic Cancer by Patient-derived Xenograft Models.

Authors - Gang Yang
View Article
mouseIHC

Angiogenesis

A novel lymphatic pattern promotes metastasis of cervical cancer in a hypoxic tumour-associated macrophage-dependent manner.

Authors - Xiao-Jing Chen
View Article
ratIHC,IF

Proc Natl Acad Sci U S A

Up-regulation of CD81 inhibits cytotrophoblast invasion and mediates maternal endothelial cell dysfunction in preeclampsia.

Authors - Li Shen
View Article
mouseIF

Cell Prolif

Insufficient GDF15 expression predisposes women to unexplained recurrent pregnancy loss by impairing extravillous trophoblast invasion

Authors - Chunzi Lyu
View Article
mouseIHC

Oncogene

USP13 promotes development and metastasis of high-grade serous ovarian carcinoma in a novel mouse model.

Authors - Juntae Kwon
View Article