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Cytokeratin 7-specific Polyclonal antibody

Cytokeratin 7-specific Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (3)

Applications

FC, IF, IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 17513-1-AP

Print datasheet

Synonyms

Cell and organelle markers, CK 7, CK7, Cytokeratin 7, Cytokeratin 7-specific, Cytoskeleton Marker, K2C7, K7, keratin 7, KRT7, Sarcolectin, SCL, Type II keratin Kb7



经过测试的应用

Positive WB detected inA431 cells, HeLa cells, HepG2 cells, T-47D cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman lung cancer tissue, human kidney tissue, human ovary tumor tissue, human breast cancer tissue, human cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells, HeLa cells
Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy Cytokeratin 7 Ready-To-Use IHC Kit. Cytokeratin 7 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:10000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17513-1-AP targets Cytokeratin 7-specific in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human samples.

Tested Applications FC, IF, IHC, IP, WB, ELISA
Cited Applications IF, IHC, WB
Tested Reactivity human
Cited Reactivityhuman, mouse, rat, bovine
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name keratin 7
Synonyms Cell and organelle markers, CK 7, CK7, Cytokeratin 7, Cytokeratin 7-specific, Cytoskeleton Marker, K2C7, K7, keratin 7, KRT7, Sarcolectin, SCL, Type II keratin Kb7
Calculated Molecular Weight 51 kDa
Observed Molecular Weight 51 kDa
GenBank Accession NumberNM_005556
Gene Symbol KRT7
Gene ID (NCBI) 3855
RRIDAB_2134468
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP08729
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. KRT7 is a type II keratin. It is specifically expressed in the simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vessels. This antibody is specifically against KRT7.

实验方案

Product Specific Protocols
WB protocol for Cytokeratin 7-specific antibody 17513-1-APDownload protocol
IHC protocol for Cytokeratin 7-specific antibody 17513-1-APDownload protocol
IF protocol for Cytokeratin 7-specific antibody 17513-1-APDownload protocol
IP protocol for Cytokeratin 7-specific antibody 17513-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratIF

Transl Res

SERPINA5 may promote the development of preeclampsia by disruption of the uPA/uPAR pathway.

Authors - Yan Long
humanIHC

Clin Cancer Res

Integrative Genomic Analysis of Gemcitabine Resistance in Pancreatic Cancer by Patient-derived Xenograft Models.

Authors - Gang Yang
mouseIHC

Angiogenesis

A novel lymphatic pattern promotes metastasis of cervical cancer in a hypoxic tumour-associated macrophage-dependent manner.

Authors - Xiao-Jing Chen
ratIHC,IF

Proc Natl Acad Sci U S A

Up-regulation of CD81 inhibits cytotrophoblast invasion and mediates maternal endothelial cell dysfunction in preeclampsia.

Authors - Li Shen
mouseIF

Cell Prolif

Insufficient GDF15 expression predisposes women to unexplained recurrent pregnancy loss by impairing extravillous trophoblast invasion

Authors - Chunzi Lyu
mouseIHC

Oncogene

USP13 promotes development and metastasis of high-grade serous ovarian carcinoma in a novel mouse model.

Authors - Juntae Kwon