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Lamin B1 Monoclonal antibody
Lamin B1 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse, rat and More (5)
Applications
WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA and More (2)
Conjugate
Unconjugated
371
CloneNo.
3C10G12
验证数据展示
经过测试的应用
Positive WB detected in | NCI-H1299 cells, multi-cells/tissue, HeLa cells, HepG2 cells, HEK-293 cells, Jurkat cells, K-562 cells, PC-12 cells, NIH/3T3 cells, 4T1cells |
Positive IP detected in | HeLa cells |
Positive IHC detected in | human pancreas cancer tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | mouse eye tissue |
Positive IF/ICC detected in | HepG2 cells, HeLa cells |
Positive FC (Intra) detected in | HeLa cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:20000-1:100000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:250-1:1000 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
66095-1-Ig targets Lamin B1 in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA Application Description |
Cited Applications | WB, IHC, IF, IP, CoIP |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat, rabbit, chicken, zebrafish, bovine, hamster |
Immunogen | Lamin B1 fusion protein Ag20522 种属同源性预测 |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Full Name | lamin B1 |
Synonyms | LMNB1, LMNB, LMN2, Lamin-B1, laminB1 |
Calculated Molecular Weight | 66 kDa |
Observed Molecular Weight | 66-70 kDa |
GenBank Accession Number | BC012295 |
Gene Symbol | Lamin B1 |
Gene ID (NCBI) | 4001 |
ENSEMBL Gene ID | ENSG00000113368 |
RRID | AB_11232208 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P20700 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for Lamin B1 antibody 66095-1-Ig | Download protocol |
IHC protocol for Lamin B1 antibody 66095-1-Ig | Download protocol |
IF protocol for Lamin B1 antibody 66095-1-Ig | Download protocol |
IP protocol for Lamin B1 antibody 66095-1-Ig | Download protocol |
FC protocol for Lamin B1 antibody 66095-1-Ig | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Nat Cell Biol Ceramide-rich microdomains facilitate nuclear envelope budding for non-conventional exosome formation. | ||
J Hepatol OGDHL silencing promotes hepatocellular carcinoma by reprogramming glutamine metabolism. | ||
Cell Rep Med Management of prostate cancer by targeting 3βHSD1 after enzalutamide and abiraterone treatment. | ||
Nat Commun ARF1 prevents aberrant type I interferon induction by regulating STING activation and recycling | ||
Nat Commun FOXP3+ regulatory T cell perturbation mediated by the IFNγ-STAT1-IFITM3 feedback loop is essential for anti-tumor immunity | ||
J Clin Invest FAM117B promotes gastric cancer growth and drug resistance by targeting the KEAP1/NRF2 signaling pathway |