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  • Featured Product
  • KD/KO Validated

SAPK4 Polyclonal antibody

SAPK4 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

IHC, WB,ELISA

Conjugate

Unconjugated

Cat No : 10217-1-AP

Print datasheet

Synonyms

MAP kinase 13, MAP kinase p38 delta, MAPK 13, MAPK13, p38delta, PRKM13, SAPK4

验证数据展示

  • IHC staining of human pancreas cancer using 10217-1-AP

    IHC staining of human pancreas cancer using 10217-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10217-1-AP (SAPK4 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human pancreas cancer using 10217-1-AP

    IHC staining of human pancreas cancer using 10217-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10217-1-AP (SAPK4 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis using 10217-1-AP

    WB analysis using 10217-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10217-1-AP (SAPK4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inU-937 cells, HEK-293 cells, HeLa cells, HepG2 cells
Positive IHC detected inhuman pancreas cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10217-1-AP targets SAPK4 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications IHC, WB,ELISA
Cited ApplicationsWB, IHC
Tested Reactivity human
Cited Reactivityhuman, rat, hamster
Immunogen SAPK4 fusion protein Ag0255 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name mitogen-activated protein kinase 13
Synonyms MAP kinase 13, MAP kinase p38 delta, MAPK 13, MAPK13, p38delta, PRKM13, SAPK4
Calculated Molecular Weight 38 kDa
Observed Molecular Weight 42 kDa
GenBank Accession NumberBC000433
Gene Symbol MAPK13
Gene ID (NCBI) 5603
RRIDAB_2877727
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO15264
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SAPK4 antibody 10217-1-APDownload protocol
IHC protocol for SAPK4 antibody 10217-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
hamsterWB

Sci Rep

Cellular response to persistent foot-and-mouth disease virus infection is linked to specific types of alterations in the host cell transcriptome.

Authors - Lingling Han
View Article
humanWB,IHC

Tumour Biol

Clinicopathological significance of p38β, p38γ, and p38δ and its biological roles in esophageal squamous cell carcinoma.

Authors - Shutao Zheng
  • KD Validated
View Article
ratWB

BMC Endocr Disord

The role of MAPK11/12/13/14 (p38 MAPK) protein in dopamine agonist-resistant prolactinomas.

Authors - Shuman Wang
View Article