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验证数据展示

  • WB analysis using 17564-1-AP

    WB analysis using 17564-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17564-1-AP (CD146/MCAM antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of HepG2 using 17564-1-AP

    WB analysis of HepG2 using 17564-1-AP

    WB result of CD146/MCAM antibody (17564-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CD146/MCAM transfected HepG2 cells.

  • IHC staining of human ovary tumor using 17564-1-AP

    IHC staining of human ovary tumor using 17564-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 17564-1-AP (CD146/MCAM antibody at dilution of 1:600 (under 10x lens).

    Ready-to-use IHC kits available with CD146/MCAM antibody included
  • IHC staining of human ovary tumor using 17564-1-AP

    IHC staining of human ovary tumor using 17564-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 17564-1-AP (CD146/MCAM antibody at dilution of 1:600 (under 40x lens).

    Ready-to-use IHC kits available with CD146/MCAM antibody included
  • IF Staining of mouse spleen using 17564-1-AP

    IF Staining of mouse spleen using 17564-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using CD146/MCAM antibody (17564-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse spleen using 17564-1-AP

    IF Staining of mouse spleen using 17564-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using CD146/MCAM antibody (17564-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IP experiment of HeLa using 17564-1-AP

    IP experiment of HeLa using 17564-1-AP

    IP result of anti-CD146/MCAM (IP:17564-1-AP, 4ug; Detection:17564-1-AP 1:1500) with HeLa cells lysate 4000ug.

  • IF Staining of HUVEC using 17564-1-AP

    IF Staining of HUVEC using 17564-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HUVEC cells using CD146/MCAM antibody (17564-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Featured Product
  • KD/KO验证

CD146/MCAM Polyclonal antibody

CD146/MCAM Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA
Cat No. 17564-1-AP

产品说明书

引用文献(25)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat and More (1)

应用

WB, IHC, IF/ICC, IF-P, IP, ELISA and More (2)

别名

CD146, S-endo 1 endothelial-associated antigen, MUC18, Melanoma-associated antigen MUC18, Melanoma-associated antigen A32

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, A375 cells, mouse lung tissue, HUVEC cells, HepG2 cells
Positive IP detected inHeLa cells, HepG2 cells
Positive IHC detected inhuman ovary tumor tissue, human brain tissue, human heart tissue, human kidney tissue, human lung tissue, human placenta tissue, human spleen tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse spleen tissue
Positive IF/ICC detected inHUVEC cells, HeLa cells
Planning an IHC experiment? We recommend our IHCeasy CD146/MCAM Ready-To-Use IHC Kit. CD146/MCAM primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:600
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17564-1-AP targets CD146/MCAM in WB, IHC, IF/ICC, IF-P, IP, ELISA, Cell treatment applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IF-P, IP, ELISA Application Description
文献引用应用WB, IHC, IF, IP, Cell treatment
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat, rabbit
免疫原 CD146/MCAM fusion protein Ag11762 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 melanoma cell adhesion molecule
别名 CD146, S-endo 1 endothelial-associated antigen, MUC18, Melanoma-associated antigen MUC18, Melanoma-associated antigen A32
计算分子量 646 aa, 72 kDa
观测分子量 113-120 kDa
GenBank蛋白编号BC056418
基因名称 CD146
Gene ID (NCBI) 4162
RRIDAB_2143373
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP43121
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD146, also known as melanoma cell adhesion molecule (MCAM) or MUC18, originally identified as a biomarker of melanoma progression, is a transmembrane glycoprotein of 113-130 kDa, belonging to the immunoglobulin (Ig) superfamily (PMID: 8378324; 25993332). Structurally, it consists of five Ig domains, a transmembrane domain, and a cytoplasmic region. In normal adult tissue, CD146 is primarily expressed by vascular endothelium and smooth muscle. CD146 is a key cell adhesion protein in vascular endothelial cell activity and angiogenesis, and has been used as marker of circulating endothelium cells (CECs) (PMID: 19356677). In addition to the membrane-anchored form of CD146, a soluble form of CD146 (sCD146, 105 kDa) has also been found in human plasma and in the supernatant of cultured human endothelial cells (PMID: 9462829; 19229070; 16374253; 14597988).

实验方案

Product Specific Protocols
WB protocol for CD146/MCAM antibody 17564-1-APDownload protocol
IHC protocol for CD146/MCAM antibody 17564-1-APDownload protocol
IF protocol for CD146/MCAM antibody 17564-1-APDownload protocol
IP protocol for CD146/MCAM antibody 17564-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIHC

Biomaterials

Macropore design of tissue engineering scaffolds regulates mesenchymal stem cell differentiation fate.

Authors - W Benton Swanson
View Article
ratIF

Adv Healthc Mater

Extrafibrillarly Demineralized Dentin Matrix for Bone Regeneration

Authors - Xiaoyi Wu
View Article
humanWB

Oncogene

The histone demethylase KDM3A, and its downstream target MCAM, promote Ewing Sarcoma cell migration and metastasis.

Authors - M Sechler
  • KD Validated
View Article
humanIF

Cell Prolif

Dental pulp stem cells-based therapy for the oviduct injury via immunomodulation and angiogenesis in vivo.

Authors - Lihua Luo
View Article
humanFC

Stem Cell Res Ther

Connective tissue growth factor promotes cementogenesis and cementum repair via Cx43/β-catenin axis

Authors - Zuping Wu
View Article
ratWB

J Proteome Res

Comparative Proteomic Analysis of Primary Schwann Cells and a Spontaneously Immortalized Schwann Cell Line RSC 96: A Comprehensive Overview with a Focus on Cell Adhesion and Migration Related Proteins.

Authors - Ji Yuhua Y
  • KD Validated
View Article