• Featured Product
  • KD/KO Validated

METTL3 Polyclonal antibody

METTL3 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Monkey, Rat and More (9)

Applications

IHC, IP, WB, ELISA and More (5)

Conjugate

Unconjugated

Cat No : 15073-1-AP

Print datasheet

Synonyms

IME4, M6A, methyltransferase like 3, METTL3, MT A70, MTA70, Spo8



经过测试的应用

Positive WB detected inHEK-293 cells, HT-1080 cells, HeLa cells, mouse testis tissue, Jurkat cells, NCCIT cells, Neuro-2a cells, HepG2 cells, COS-7 cells, rat testis tissue, A549 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman renal cell carcinoma tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy METTL3 Ready-To-Use IHC Kit. METTL3 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:750-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15073-1-AP targets METTL3 in WB, RIP, IP, IHC, IF, FC, CoIP, ChIP, ELISA applications and shows reactivity with Human, Mouse, Monkey, Rat samples.

Tested Applications IHC, IP, WB, ELISA
Cited Applications ChIP, CoIP, FC, IF, IHC, IP, RIP, WB
Tested Reactivity Human, Mouse, Monkey, Rat
Cited Reactivityhuman, mouse, rat, goat, chicken, monkey, zebrafish, pig, drosophila
Immunogen METTL3 fusion protein Ag7110 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name methyltransferase like 3
Synonyms IME4, M6A, methyltransferase like 3, METTL3, MT A70, MTA70, Spo8
Calculated Molecular Weight 64 kDa
Observed Molecular Weight 65-70 kDa
GenBank Accession NumberBC001650
Gene Symbol METTL3
Gene ID (NCBI) 56339
RRIDAB_2142033
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ86U44
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

METTL3 is a key S-adenosyl-L-methionine-binding subunit, which is a component of a complex multicomponent enzyme that catalyzes the methylation of internal adenosine residues in eukaryotic mRNA, forming N6-methyladenosine. It contains 2 putative nuclear localization signals and 2 consensus methylation motifs. The calculated molecular weight of METTL3 is 64 kDa, but modified METTL3 is about 65-70 kDa.

实验方案

Product Specific Protocols
WB protocol for METTL3 antibody 15073-1-APDownload protocol
IHC protocol for METTL3 antibody 15073-1-APDownload protocol
IP protocol for METTL3 antibody 15073-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Nature

METTL3 regulates heterochromatin in mouse embryonic stem cells.

Authors - Wenqi Xu
  • KO Validated
mouseWB

Nature

The RNA m6A reader YTHDC1 silences retrotransposons and guards ES cell identity.

Authors - Jiadong Liu
  • KO Validated
humanWB

Nature

mRNA circularization by METTL3-eIF3h enhances translation and promotes oncogenesis.

Authors - Junho Choe
  • KD Validated
humanWB

Nature

N(6)-methyladenosine-dependent regulation of messenger RNA stability.

Authors - Wang Xiao X
  • KD Validated
humanWB

Mol Cancer

A positive feedback circuit driven by m6A-modified circular RNA facilitates colorectal cancer liver metastasis

Authors - Kaixuan Zeng
  • KD Validated
mouseWB,IF

Science

Stem cells. m6A mRNA methylation facilitates resolution of naïve pluripotency toward differentiation.

Authors - Shay Geula
  • KO Validated