验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, HeLa cells, HT-1080 cells, mouse testis tissue, HepG2 cells, Jurkat cells, Neuro-2a cells, A549 cells, NCCIT cells, COS-7 cells, rat testis tissue |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | human renal cell carcinoma tissue, human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells |
Planning an IHC experiment? We recommend our IHCeasy METTL3 Ready-To-Use IHC Kit. METTL3 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:750-1:3000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
15073-1-AP targets METTL3 in WB, IHC, IF/ICC, IP, CoIP, ChIP, RIP, ELISA applications and shows reactivity with human, mouse, rat, monkey samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP, CoIP, ChIP, RIP |
| 经测试反应性 | human, mouse, rat, monkey |
| 文献引用反应性 | human, mouse, rat, pig, monkey, chicken, zebrafish, hamster, goat, drosophila |
| 免疫原 | METTL3 fusion protein Ag7110 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | methyltransferase like 3 |
| 别名 | MT-A70, MTA70, MT A70, Methyltransferase-like protein 3, hMETTL3 |
| 计算分子量 | 64 kDa |
| 观测分子量 | 65-70 kDa |
| GenBank蛋白编号 | BC001650 |
| 基因名称 | METTL3 |
| Gene ID (NCBI) | 56339 |
| RRID | AB_2142033 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q86U44 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
METTL3 is a key S-adenosyl-L-methionine-binding subunit, which is a component of a complex multicomponent enzyme that catalyzes the methylation of internal adenosine residues in eukaryotic mRNA, forming N6-methyladenosine. It contains 2 putative nuclear localization signals and 2 consensus methylation motifs. The calculated molecular weight of METTL3 is 64 kDa, but modified METTL3 is about 65-70 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for METTL3 antibody 15073-1-AP | Download protocol |
| IHC protocol for METTL3 antibody 15073-1-AP | Download protocol |
| IF protocol for METTL3 antibody 15073-1-AP | Download protocol |
| IP protocol for METTL3 antibody 15073-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nature The RNA m6A reader YTHDC1 silences retrotransposons and guards ES cell identity.
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Nature mRNA circularization by METTL3-eIF3h enhances translation and promotes oncogenesis.
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Mol Cancer A positive feedback circuit driven by m6A-modified circular RNA facilitates colorectal cancer liver metastasis
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Science Stem cells. m6A mRNA methylation facilitates resolution of naïve pluripotency toward differentiation.
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