验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, PC-3 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, U-251 cells, U-87 MG cells |
| Positive IHC detected in | human prostate cancer tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
66776-1-Ig targets MFN1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.
| Tested Applications | WB, IHC, IF/ICC, ELISA Application Description |
| Cited Applications | WB, IHC, IF |
| Tested Reactivity | Human, mouse, rat |
| Cited Reactivity | human, mouse, rat, pig |
| Immunogen | MFN1 fusion protein Ag4890 种属同源性预测 |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | mitofusin 1 |
| Synonyms | DKFZp762F247, FLJ20693, Fzo homolog, hfzo1, hfzo2, MFN1, mitofusin 1, Transmembrane GTPase MFN1 |
| Calculated Molecular Weight | 741 aa, 84 kDa |
| Observed Molecular Weight | 84 kDa |
| GenBank Accession Number | BC040557 |
| Gene Symbol | MFN1 |
| Gene ID (NCBI) | 55669 |
| RRID | AB_2882122 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q8IWA4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Mitofusin-1 (MFN1) is a mediator of mitochondrial fusion. This protein and mitofusin 2 are homologs of the Drosophila protein fuzzy onion (Fzo). Mitofusins are large predicted GTPases located in outer mitochondrial membrane. They are essential for outer membrane fusion by interacting with each other to facilitate mitochondrial targeting. The mitofusins are the first known protein mediator of mitochondrial fusion, and mediate developmentally regulated post-meiotic fusion of mitochondria. Mitofusin 1 and mitofusin 2 are ubiquitinated in a PINK1/parkin-dependent manner upon induction of mitophagy(PMID: 20871098).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MFN1 antibody 66776-1-Ig | Download protocol |
| IHC protocol for MFN1 antibody 66776-1-Ig | Download protocol |
| IF protocol for MFN1 antibody 66776-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun BNIP3L/NIX-mediated mitophagy protects against glucocorticoid-induced synapse defects. | ||
Ecotoxicol Environ Saf Zinc deficiency compromises the maturational competence of porcine oocyte by inducing mitophagy and apoptosis | ||
J Cell Physiol DMT1 Maintains Iron Homeostasis to Regulate Mitochondrial Function in Porcine Oocytes | ||
Molecules Nujiangexanthone A Inhibits Cervical Cancer Cell Proliferation by Promoting Mitophagy. | ||
J Mol Histol Roles of HIF-1α/BNIP3 mediated mitophagy in mitochondrial dysfunction of letrozole-induced PCOS rats | ||
Phytomedicine Xiangsha Liujunzi Decoction improves gastrointestinal motility in functional dyspepsia with spleen deficiency syndrome by restoring mitochondrial quality control homeostasis |
