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MMP13 Polyclonal antibody

MMP13 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (4)

Applications

WB, IHC, IF/ICC, IP, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 18165-1-AP

Print datasheet

Synonyms

MMP 13, Matrix metalloproteinase-13, Matrix metalloproteinase 13, EC:3.4.24.-, Collagenase 3



经过测试的应用

Positive WB detected inHeLa cells, MCF-7 cells, mouse liver tissue, rat liver tissue, HepG2 cells, MDA-MB-453s cells
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman liver tissue, human hepatocirrhosis tissue, mouse liver tissue, human liver cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18165-1-AP targets MMP13 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB, IHC, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, pig, rabbit, zebrafish, bovine
Immunogen MMP13 fusion protein Ag12653 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name matrix metallopeptidase 13
Synonyms MMP 13, Matrix metalloproteinase-13, Matrix metalloproteinase 13, EC:3.4.24.-, Collagenase 3
Calculated Molecular Weight 471 aa, 54 kDa
Observed Molecular Weight 65-70 kDa, 50-55 kDa, 40 kDa
GenBank Accession NumberBC074808
Gene Symbol MMP13
Gene ID (NCBI) 4322
RRIDAB_2144858
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP45452
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MMP-13(Matrix metalloproteinase-13), cleaves type I collagen and was previously thought to be the rodent analogue of human interstitial MMP-1. MMP-13 is found in hypertrophic and calcifying cartilage of mammalian growth plate. The bands seen with rat MMP-13 are consistent with a 59-kDa latent form and a 43-kDa active form reported for this enzyme, along with a smaller band at approximately 30 kDa(PMID:10771523). It also can be detected 3 bands that the glycosylated proMMP-13 (70 kDa), unglycosylated proform (55 kDa), and active MMP-13 enzyme (40kDa) by western blot(PMID:18332263).

实验方案

Product Specific Protocols
WB protocol for MMP13 antibody 18165-1-APDownload protocol
IHC protocol for MMP13 antibody 18165-1-APDownload protocol
IF protocol for MMP13 antibody 18165-1-APDownload protocol
IP protocol for MMP13 antibody 18165-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,mouseIF

Nature

A plant-derived natural photosynthetic system for improving cell anabolism

Authors - Pengfei Chen
human,mouseWB,IF

Sci Transl Med

Single-cell atlas of human infrapatellar fat pad and synovium implicates APOE signaling in osteoarthritis pathology

Authors - Su'an Tang
human,mouseWB,IF

Adv Sci (Weinh)

Inflammatory Fibroblast-Like Synoviocyte-Derived Exosomes Aggravate Osteoarthritis via Enhancing Macrophage Glycolysis

Authors - Bin Liu
mouse,ratWB,IF

Exp Mol Med

SIRT5-related desuccinylation modification of AIFM1 protects against compression-induced intervertebral disc degeneration by regulating mitochondrial homeostasis

Authors - Jianxin Mao
mouseIHC

Mol Ther

Hnrnpk Protects Against Osteoarthritis through Targeting WWC1 mRNA and Inhibiting Hippo Signaling Pathway

Authors - Shun Zhang
mouseWB,IHC

Nat Commun

Dual functions of microRNA-17 in maintaining cartilage homeostasis and protection against osteoarthritis.

Authors - Yun Zhang