MMP9 (Middle) Polyclonal antibody
MMP9 (Middle) Polyclonal Antibody for WB, IP, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (1)
Applications
WB, IP, IHC, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | human saliva tissue |
Positive IP detected in | human saliva tissue |
Positive IHC detected in | human tonsillitis tissue, human lung cancer tissue, human spleen tissue, mouse liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
27306-1-AP targets MMP9 (Middle) in WB, IHC, IP, ELISA, IF applications and shows reactivity with human, mouse samples.
Tested Applications | WB, IP, IHC, ELISA Application Description |
Cited Applications | WB, IHC, IF |
Tested Reactivity | human, mouse |
Cited Reactivity | human, mouse, rat |
Immunogen | MMP9 (Middle) fusion protein Ag26132 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase) |
Synonyms | 92 kDa gelatinase, 92 kDa type IV collagenase, CLG4B, Gelatinase B, GELB, Matrix metalloproteinase 9, MMP 9, MMP9, MMP9 (Middle) |
Calculated Molecular Weight | 707 aa, 78 kDa |
Observed Molecular Weight | 92 kDa |
GenBank Accession Number | BC006093 |
Gene Symbol | MMP9 |
Gene ID (NCBI) | 4318 |
RRID | AB_2880837 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P14780 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for MMP9 (Middle) antibody 27306-1-AP | Download protocol |
IHC protocol for MMP9 (Middle) antibody 27306-1-AP | Download protocol |
IP protocol for MMP9 (Middle) antibody 27306-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
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Int J Mol Sci Comprehensive Analysis of MICALL2 Reveals Its Potential Roles in EGFR Stabilization and Ovarian Cancer Cell Invasion | ||
Front Endocrinol (Lausanne) Nicotinamide mononucleotide attenuates HIF-1α activation and fibrosis in hypoxic adipose tissue via NAD+/SIRT1 axis | ||
Int J Biol Sci TDG suppresses the migration and invasion of human colon cancer cells via the DNMT3A/TIMP2 axis. | ||
Front Mol Biosci Comprehensive Characterization of Ageing-Relevant Subtypes Associated With Different Tumorigenesis and Tumor Microenvironment in Prostate Cancer. | ||
Front Mol Biosci miR-193a-3p Mediates Placenta Accreta Spectrum Development by Targeting EFNB2 via Epithelial-Mesenchymal Transition Pathway Under Decidua Defect Conditions. | ||
Front Mol Biosci CUX1 Facilitates the Development of Oncogenic Properties Via Activating Wnt/β-Catenin Signaling Pathway in Glioma. |